甜菜红色食品染料可以通过工程Yarrowia lipolytica更可持续地生产

相关实验视频

Updated: Jun 5, 2026

10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

13.8K

Philip Tinggaard Thomsen¹, Samir Meramo¹, Lorenzo Ninivaggi¹

¹The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark.

Nature microbiology

|November 29, 2023

概括

这项研究设计了酵母,通过发酵产生天然的红色食品染料 (贝塔宁),为甜菜提取提供了一个可持续的替代方案. 这种生物技术方法显著减少了土地利用和资源消耗.

科学领域:

生物技术是生物技术.
食品科学食品科学食品科学
合成生物学合成生物学

背景情况:

由于安全和可持续性问题,消费者对天然食品色素的需求正在增加.
贝塔宁是一种天然的红色食品染料,来自甜菜,产量低,提取成本高.
目前的甜菜提取方法资源密集,产生大量的废物.

研究的目的:

开发一种可持续的生物技术工艺,用于生产贝他林 (贝他林和异贝他林).
为了改造油性酵母Yarrowia lipolytica以提高贝他林的生产.
与传统提取相比,评估发酵过程的环境和经济可行性.

主要方法:

雅罗维亚脂质菌的代谢工程用于贝他林生物合成.
使用葡萄糖作为碳来源优化发酵条件 (食批量).
工业规模生产的生命周期评估 (LCA) 和技术经济评估 (TEA).

主要成果:

在51小时内实现了1,271±141 mg/L贝坦宁和55±7 mg/L异贝坦宁的高产量.
与工业规模的甜菜提取相比,发酵过程的土地,能源和资源需求显著降低.
技术经济评估表明,发酵贝塔宁生产的经济可行性.

结论:

酵母发酵为天然红色食品染料生产提供了一种可持续且潜在的成本效益高的替代方案.
贝塔宁的生物技术生产解决了传统甜菜提取的局限性.
工程化Yarrowia lipolytica为工业规模的天然染料制造提供了一个可行的平台.

相关概念视频

01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

01:23

Red Algae

Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...

01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

01:18

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...