卵巢ERβ细胞组和转录组显示染色质与LRH-1的相互作用
Madeleine Birgersson1,2, Rajitha Indukuri1, Linnéa Lindquist1,2
1Science for Life Laboratory (SciLifeLab), Department of Protein Science, KTH Royal Institute of Technology, 171 21, Solna, Sweden.
BMC biology
|November 29, 2023
概括
雌激素受体β (ERβ) 对卵巢功能至关重要,但其机制尚不清楚. 这项研究揭示了ERββ的存在.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 基因组学就是基因组学.
背景情况:
- 雌激素受体β (ERβ,Esr2) 对卵巢过程如卵泡生成和排卵至关重要.
- 控制ERβ在卵巢中的作用的精确分子机制在很大程度上仍未被描述.
研究的目的:
- 为了阐明ERβ在小鼠卵巢中的全基因组功能.
- 识别直接的ERβ目标基因并了解其调节网络.
- 研究ERβ与肝受体同源1 (LRH-1) 之间的相互作用.
主要方法:
- 在野生型和ERβ淘汰赛小鼠卵巢上进行了ERβChIP测序和RNA测序.
- 集成的细胞组和转录组数据以识别ERβ目标和功能.
- 使用了细胞类型解卷分析和ChIP-reChIP测试.
- 进行的交易活化试验研究ERβ和LRH-1相互作用.
主要成果:
- 确定了ERβ对参与发育,细胞迁移,脂质代谢和对缺氧和雌激素反应的基因的显著影响.
- 失去了ERβ改变了卵巢细胞的比例,减少了白质细胞,增加了theca和累积细胞.
- 证明了ERβ和LRH-1在共享的染色质位置的广泛联合结合,特别是在颗粒细胞中.
- 表明ERβ和LRH-1可以相互抑制转录活性.
结论:
- 在卵巢中表征了全基因组的ERβ染色体结合和基因调节.
- 揭示了ERβ和LRH-1之间的广泛交叉通话,提供了机械学的见解.
- 建立了理解ERβ在卵巢生理学和生育能力中的作用的基础.
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