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在批量和料批量脂质生产过程中,工程Rhodosporidium toruloides对水解盐的耐受性
William Woodruff1,2, Narendra Naik Deshavath3,2, Vionna Susanto1,2
1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, USA.
Biotechnology for biofuels and bioproducts
|November 29, 2023
概括
高酸盐缓冲度不会抑制工程化Rhodosporidium toruloides的生长或从基纤维素原料中生产脂质. 减少酸盐缓冲剂可以节省酶解水解过程中的成本,提供更可持续的生物工艺.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 可持续化学 可持续化学
背景情况:
- 油性酵母可以可持续地将纤维素生物质转化为燃料和化学品.
- 预处理和水解条件可以抑制酵母菌在纤维素基质上的生长.
- 高酸盐缓冲度可能会抑制Saccharomyces cerevisiae的下游发酵.
研究的目的:
- 为了研究酸盐缓冲度对工程Rhodosporidium toruloides生长和脂质生产的作用,使用水解糖.
- 为了确定降低酸盐缓冲度是否会影响酶化水解期间的糖产量.
主要方法:
- 在不同酸盐缓冲度下,在水解液上培育一种工程R. toruloides菌株.
- 在高酸盐和低酸盐条件下比较细胞生长率,糖利用率和脂质生产率.
- 分析酶化水解中的糖回收和度.
主要成果:
- 在高酸盐和低酸盐缓冲条件下观察到类似的糖回收率和度.
- 在批发发酵过程中,两种酸盐度之间没有发现细胞生长,糖利用率或脂质生产率的显著差异.
- 在用低酸盐水解剂养批量生长过程中,脂质标位达到16.7 g/L.
结论:
- 酸盐缓冲度没有抑制工程R. toruloides菌株的生长或脂质生产.
- 在酶性水解过程中降低酸盐缓冲度不会对糖产量产生负面影响.
- 在扩展过程中使用较低的酸盐缓冲度可以节省大量成本 (每生物质131美元).
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