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在癌症中微RNA治疗的可视化,由小细胞外囊泡传递

Peiwen Fu^1,2, Yumeng Guo¹, Yanan Luo¹

¹Jiangsu Province Key Laboratory of Medical Science and Laboratory Medicine, Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

Journal of nanobiotechnology

|November 30, 2023

概括

携带石墨烯量子点和miR-193a-3p的小细胞外囊泡 (sEV) 能够实时跟踪基因疗法. 这种疗效平台增强了癌症的亡,并在体内评估治疗的疗效.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
在瘤学瘤学.

背景情况:

细胞外囊泡 (EV) 介导的微RNA (miRNA) 输送显示出癌症治疗的前景.
在体内对miRNA-mRNA杂交的成像对于评估治疗结果至关重要.
目前的方法很难实时可视化基因疗法效应.

研究的目的:

开发一个纳米尺度的光"关闭"复合体,用于实时可视化基因疗法.
在胃癌模型中评估miRNA输送和治疗效果的效率.
为增强癌症治疗创建一个超神论平台.

主要方法:

设计了一个由石墨烯量子点 (GQD) 和miR-193a-3p与光标签 (Cy5-miR) 结合的复合体.
使用超声波将GQDs/Cy5-miR复合体封装成小细胞外囊泡 (sEVs).
使用"关闭"光系统在体外和体内监测miRNA-mRNA结合 (miR-193a-3p和CCND1).

主要成果:

该GQDs/Cy5-miR@sEVs系统在miRNA-mRNA结合时成功激活了"启动"光信号.
sEVs在体内延长了保留时间,这表明miRNA降解减少.
增强的细胞吸收GQDs/Cy5-miR@sEVs导致显著的癌症亡在体外和体内.

结论:

GQDs/Cy5-miR@sEVs作为一个有效的疗效平台,用于实时监测基因疗法.
这种方法使得有效的miRNA输送和评估治疗癌症的疗效.
开发的平台具有精细癌症基因治疗策略的潜力.

关键词:

癌症治疗治疗癌症治疗细胞外囊泡中的细胞外囊泡.基因输送基因输送基因输送量子点是一个量子点.这是一个微型RNA.

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MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...