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单细胞分析揭示了结直肠癌微环境中巨细胞的激活

Zhenyu Xie¹, Liaoran Niu¹, Gaozan Zheng¹

¹The State Key Laboratory of Cancer Biology, Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, 169 Changle Road, Xi'an, Shaanxi, 710032, China.

Cell & bioscience

|November 30, 2023

概括

巨细胞 (MCs) 在结直肠癌 (CRC) 中的密度较低,但会被激活. 这种激活可能是由瘤微环境因素驱动的,与CRC患者的更好的预后相关.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

巨细胞 (MCs) 在结直肠癌 (CRC) 发病过程中的作用尚未完全理解.
在CRC微环境中缺乏对MC的全面单细胞分析.

研究的目的:

在CRC中研究MCs的异质性和功能影响,使用多omics方法.
识别MC特征及其与CRC临床结果的相关性.

主要方法:

单细胞测序,空间转录组学和大量组织测序的整合.
鉴定了五个关键的MC特征基因 (TPSAB1,TPSB2,CPA3,HPGDS,MS4A2).这些基因的特征包括:
分析MC密度,激活状态和与其他细胞类型的同定位.

主要成果:

与正常组织相比,CRC中MC密度降低.
在CRC中,MCs表现出激活的表型,其特点是特征基因和调解者的表达更高.
与激活的MCs相关的独特的MC签名预测了CRC和胰腺癌队列中更好的预后.
瘤相关纤维细胞和内皮细胞中KITLG表达的升高表明通过KITLG-KIT轴激活MCs.

结论:

大肠直肠癌的特点是MC密度下降,但活性MC表型.
瘤微环境中的KITLG-KIT轴可能在MC激活中发挥作用,并可能抑制瘤进展.
这些发现提供了对MC在CRC中复杂作用的见解,并建议潜在的治疗点.

关键词:

激活活动激活活动结肠直肠癌是一种癌症.异质性异质性异质性巨细胞是巨细胞.预后预后预测预测一个单细胞分析.

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