在有意识的Suncus murinus中,自主神经系统参与结肠收缩
Miu Suzuki1, Ayumi Watanabe1, Jin Huang1
1Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Japan.
Neurogastroenterology and motility
|November 30, 2023
概括
迷走神经和交感神经在Suncus murinus中没有启动结肠巨型迁移收缩 (GMCs). 然而,这些自主神经调节GMC的频率,影响结肠运动.
科学领域:
- 神经胃肠病学 神经胃肠病学
- 自主神经系统的调节规则
- 胃肠道运动性 胃肠道运动性
背景情况:
- 结肠运动受肠-大脑轴的影响,但确切的机制尚不清楚.
- 自主神经系统在结肠功能中的作用需要进一步研究.
- 桑库斯 (Suncus murinus) 提供了一个适合研究胃肠机动性的模型.
研究的目的:
- 研究自主神经系统在调节结肠运动中的参与.
- 探索阴道和交感神经在结肠巨型迁移收缩 (GMCs) 中的作用.
主要方法:
- 同时24小时记录结肠机动性,食和排便在经过vagotomized和reserpine处理的Suncus murinus中.
- 对于氨酸氧酶 (TH) 的免疫组织化学和在阳大脑中对皮质otropin释放激素 (CRH) 的 in situ 杂交.
- 评估c-Fos表达在对结直肠膨胀的反应中,以确定神经元激活.
主要成果:
- 植物学导致集群的GMCs,其中增加的百分比发生在没有排便的情况下.
- 服用Reserpine改变了GMC的频率,特别是在光明和黑暗时期,并且增加了没有排便的GMC.
- 结肠直肠膨胀诱导了神经元位置的c-Fos表达,这表明了交感通路的参与.
结论:
- 迷走神经和交感神经在体内启动GMC并不是必不可少的.
- 自主神经可能在控制Suncus murinus中的GMC频率方面发挥调节作用.
- 这些发现有助于理解大肠运动的神经自主控制.
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