病毒性基因特征和Salmonella的抗菌敏感性从的布兰卡斯特
Evie Khoo1,2, Roseliza Roslee2, Zunita Zakaria1,3
1Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Journal of veterinary science
|November 30, 2023
概括
来自马来西亚的多药耐药沙门氏菌Brancaster菌株具有显著的毒性基因. 这一发现凸显了沙门氏菌作为一种潜在的食物传播动物性传染病威胁,需要进一步的监测和控制措施.
科学领域:
- 食品传播病原体研究 食品传播病原体研究
- 抗微生物耐药性研究研究
- 禽类微生物学 家禽微生物学
背景情况:
- 传统的沙门氏菌血型鉴定缺乏对病原性的洞察力.
- 沙门氏菌 (Salmonella enterica) 亚种的流行率正在上升. 在马来西亚的家禽养殖场观察到的 enterica serovar Brancaster.
- 需要了解S. Brancaster的毒性和耐药性概况.
研究的目的:
- 为了研究从马来西亚中分离的S. Brancaster中的毒性决定因素.
- 评估这些S. Brancaster分离物中的抗菌素耐药性模式.
主要方法:
- 从样本 (2017-2022) 中分析了100种已存档的S.Brancaster菌株.
- 多重复合PCR用于检测八个关键的沙门氏菌毒性基因 (invA,sipB,sifa,cdtB,sitC,spiA,spvB,sopB).
- 通过盘扩散对九种抗生素进行抗菌敏感性测试;PCR检测ACSSuT耐药菌株中的耐药基因.
主要成果:
- 所有八个向病毒性基因 (invA, sitC, spiA, sipB, sopB, sifA, cdtB, spvB) 都在S. Brancaster分离物中被检测到.
- 36种不同的抗生素图案显示出高的多药性耐药性,其中安培素耐药性最为普遍.
- 超过三分之一的分离物显示出ACSSuT耐药性,并确定了七个特定的耐药性基因 (bla, floR, strA, ant(3′′) -Ia, sul-1, sul-2, tetA).
结论:
- 从中分离出来的S.Brancaster菌具有全面的毒性基因组,与侵袭性非类风型沙门氏菌血清菌类相比.
- 由于存在多种毒性因子和显著的抗微生物耐药性,S. Brancaster被归类为一种值得注意的食源性动物病原体.
- 调查结果强调了监测和管理家禽中的S. Brancaster的重要性,以减轻公共卫生风险.
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