可注射的神经水凝作为in vivo治疗性输送车辆
Nora Hlavac1, Deanna Bousalis1, Emily Pallack1
1Biomedical Engineering, University of Florida, Gainesville, FL, US.
Regenerative engineering and translational medicine
|November 30, 2023
概括
可以注射的外周神经 (iPN) 水凝在体内成功输送,形成了凝支架. 在PLGA中封装治疗药物延长了它们的释放时间,显示出再生疗法的前景.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 外围神经组织工程旨在开发用于神经修复的功能性支架.
- 可注射的水凝提供了最少侵入性的治疗输送选择.
- 脱细胞化组织支架可以保留本地细胞外基质线索.
研究的目的:
- 为了在体内证明可注射的外周神经 (iPN) 水凝的输送和凝.
- 为了研究从iPN水凝中控制释放生物分子有效载荷.
- 探索将iPN水凝与脂肪衍生干细胞 (ASC) 秘密组结合起来,以实现再生疗法的潜力.
主要方法:
- 鼠的外周神经组织被脱细胞化,以创建iPN水凝前体.
- 通过皮下注射iPN溶液给Sprague Dawley大鼠,并使用体内成像系统 (IVIS) 进行可视化.
- 用德克斯染料和PLGA封装的德克斯染料来评估释放动力学;ASC分泌基因被分析成长因子并封装在PLGA中.
主要成果:
- 取得了成功的皮下注射和iPN水凝的体内凝.
- 与自由染料相比,PLGA封装显著延长了从iPN水凝中释放德克斯染料的时间.
- 在介质刺激时,ASC分泌体表现出高调的VEGF分泌,最大的PLGA负载效率为28%.
结论:
- 可注射的iPN水凝配方可用于体内输送和支架形成.
- PLGA封装提供了一种控制和延长iPN水凝中治疗分子释放的方法.
- 对于未来的组合疗法,iPN水凝具有潜在的潜力,利用像干细胞分泌体这样的再生生物分子剂.
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