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与大祖黑山羊骨肌肉发育相关的候选circRNAs
Chengli Liu1, Pu Yang1, Xiao Wang1
1College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China.
Animal biotechnology
|November 30, 2023
概括
这项研究确定了在山羊中调节骨肌肉发育 (SMD) 的关键循环RNA (circRNAs). 研究结果揭示了差异表达的circRNA及其宿主基因,为肌肉生长遗传学提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
背景情况:
- 循环RNAs (circRNAs) 是已知的影响骨肌肉发育 (SMD) 的非编码RNAs.
- 肌肉细胞中circRNA调节的精确分子机制尚未完全理解.
- 研究山羊肌肉发育中的circRNAs对于理解肌肉生长至关重要.
研究的目的:
- 为了确定参与山羊骨肌肉发育的关键circRNAs.
- 阐明肌肉生长过程中circRNA调节的分子遗传基础.
- 分析羊肉肌肉发育中的circRNAs的表达模式和功能作用.
主要方法:
- 用RNA测序 (RNA-seq) 来分析不同发育阶段 (胚胎第75天和产后第1天) 的山羊长脊肌中的circRNA表达.
- 使用定量实时PCR (RT-qPCR) 和桑格测序来验证差异表达circRNAs (DEcircRNAs) 的表达和真实性.
- 生物信息学分析,包括基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析,在DEcircRNAs的宿主基因上进行.
主要成果:
- 在发育阶段之间确定了140个显著差异表达的circRNAs (DEcircRNAs).
- 在116个与DEcircRNAs相关的宿主基因 (HGs) 中,76个也显著差异地表达.
- 验证通过RT-qPCR证实了10个DEcircRNA的80%一致性,并通过桑格测序验证了7个DEcircRNA的真实性.
- 功能注释显示了74个HG的845个GO条款和35个HG的85个KEGG路径的丰富.
结论:
- 这项研究提供了在山羊骨肌肉发育过程中DEcircRNA及其宿主基因的全面目录.
- 这些发现提供了关于circRNA介导肌肉生长调节的遗传基础的见解.
- 这项研究为未来关于特定circRNAs在肌肉生物学中的功能作用的研究奠定了基础.
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