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艾伦氧化物合成酶1有助于限制谷物积累和米中幼苗排毒

Xin Fan¹, Haiyang Tang¹, Xuan Chen¹

¹MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, College of Agriculture, Yangtze University, Jingzhou, 434025, China.

|November 30, 2023

概括

雅斯蒙酸 (JA) 生物合成酶OsAOS1和OsAOS2对 (As) 在米中的积累和耐受性产生影响. OsAOS1影响As转位和解毒,而OsAOS1和OsAOS2对于As耐受性至关重要.

科学领域:

植物生物学植物生物学
环境科学环境科学
生物化学生物化学

背景情况:

(As) 是一种有毒的金属化物,在米等作物中积累.
素酸 (JA) 对于植物生长和应激反应至关重要.
内源JA在植物 (As) 代谢中的作用尚不清楚.

研究的目的:

研究JA生物合成酶Allene氧化合成酶 (OsAOS1和OsAOS2) 在 (As) 积累和耐受性中的功能.
阐明AOS1和AOS2基因的进化起源.

主要方法:

对AOS基因演变的生物信息分析.
在压力下对大米组织的基因表达分析.
功能丧失的osaos1和osaos2突变体的特征.
对转位,基因表达和活性氧物种 (ROS) 水平的分析.

主要成果:

OsAOS1和OsAOS2是从菌藻类进化而来的.
OsAOS1和OsAOS2是高度表达的,并由大米中的诱导.
osaos1-1突变体表现出谷物的度增加,这是由于酸盐的根到芽转移[As(V) ]的增强.
无论是osaos1-1还是osaos2-1突变,都对酸盐[As(V]和酸盐[As(III]的敏感性增加.
突变破坏了关键相关基因 (OsPT4,OsNIP3;2,OsOASTL-A1) 的表达,并改变了ROS的稳态.

结论:

OsAOS1在调节分配和大米中排毒方面发挥着重要作用,部分是通过基因表达和ROS恒温.
OsAOS2对于保持大米对压力的耐受性至关重要.
这些发现凸显了JA生物合成在植物管理中的重要性.

关键词:

对于的耐受性进化生物信息学是一种进化生物信息学.雅斯蒙酸是什么意思雅斯蒙酸是什么意思米树 (Oryza sativa) L. 米树 (Oryza sativa) L. 米树 (Oryza sativa) 是一个植物.在ROS的恒常性中,ROS的恒常性形成.

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