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Updated: Jul 9, 2025

Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
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Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria

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便宜的显微镜可能会为大众带来蛋白质映射

Eric Hand

    Science (New York, N.Y.)
    |November 30, 2023
    PubMed
    概括

    研究人员开发了一种新型,便宜的测定蛋白质结构的装置. 这种结构生物学突破可以加速药物发现和蛋白质工程.

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    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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