来自Pseudomonas spp. 的杀虫蛋白的相互作用. 和Bacillus thuringiensis用于虫管理
Jardel Diego Barbosa Rodrigues1, Raquel Oliveira Moreira1, Jackson Antônio Marcondes de Souza1
1Biology Department, Faculty of Agrarian and Veterinary Sciences (Jaboticabal Campus), São Paulo State University (UNESP), São Paulo, Brazil.
PloS one
|November 30, 2023
概括
这项研究探讨了Pseudomonas和Bacillus thuringiensis (Bt) 蛋白对棉花虫 (Anthonomus grandis) 的有效性. 结合这些杀虫剂蛋白质显著提高了毒性,为棉花的可持续害虫管理提供了一种战略.
科学领域:
- 农业科学 农业科学
- 昆虫学 昆虫学是一门学科.
- 微生物生物技术 微生物生物技术
背景情况:
- 棉花作物的产量受到主要害虫Anthonomus grandis的重大影响.
- 甲杆菌 (Bt) 蛋白质提供昆虫害虫控制,但需要不同的方法来维持它们的有效性.
- 正在探索 Pseudomonas 种类,因为它们的 coleopteran 杀虫特性.
研究的目的:
- 为了评估Pseudomonas spp.的毒性. 蛋白质 (IPD072Aa,PIP-47Aa) 单独或与B. thuringiensis (Bt) 蛋白质 (Cry1Ia10,Cry3Aa,Cry8B) 结合使用.
- 评估这些蛋白质对棉花害虫Anthonomus grandis的有效性.
- 确定不同杀虫剂蛋白之间的协同相互作用,以加强害虫控制.
主要方法:
- 合成和克隆了IPD072Aa和PIP-47Aa蛋白质的基因.
- 再组合的大肠杆菌克隆被用于生产Cry1Ia10,Cry3Aa和Cry8B蛋白质.
- 蛋白质被量化并纳入人工饮食中,用于致命度 (LC) 生物测试,包括单独和组合治疗.
主要成果:
- 所有测试的蛋白质 (IPD072Aa,PIP-47Aa,Cry1Ia10,Cry3Aa,Cry8B) 都显示出对A. grandis的个体疗效,Cry8B是最有毒的.
- 在Cry1Ia10和Cry3Aa之间观察到显著的协同作用.
- 涉及Cry3Aa和PIP-47Aa和其他蛋白质的组合显示出明显的协同效应,增加了对新生幼虫的毒性.
结论:
- 来自Pseudomonas spp. 的杀虫剂蛋白质的组合. 和B. thuringiensis增强了对棉花虫的毒性.
- 这些发现表明,棉花植物中的金字塔基因有可能改善害虫管理.
- 开发新的蛋白质组合可以延长杀虫技术在棉花种植中的有效性.
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