Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.4K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.4K
Operons02:09

Operons

49.1K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
MOSFET: Enhancement Mode01:22

MOSFET: Enhancement Mode

346
Enhancement-mode MOSFETs are pivotal components in electronics, distinguished by their capacity to act as highly efficient switches. They are part of the larger family of metal-oxide Semiconductor Field-Effect Transistors (MOSFETs). They are available in two types: p-channel and n-channel, each tailored to specific polarity operations.
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no...
346
What is Genetic Engineering?00:49

What is Genetic Engineering?

74.2K
Overview
74.2K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

917
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
917

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.

Engineering microbiology·2026
Same author

Comparative Analysis of Gut Microbiome Dynamics and Dietary Shifts in Three Pollinator Species During Alfalfa Pollination: Insights from Environmental DNA Metabarcoding.

Insects·2026
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

Engineering an Extremely Hybrid PKS for Adipic Acid Production.

ACS synthetic biology·2026
Same author

The impact of plant restoration on soil aggregates in karst mountainous areas of southwestern China.

iScience·2026
Same author

Determinants of childhood and adolescent obesity: An explainable AI approach using the ICAD database.

Public health·2026

相关实验视频

Updated: Jul 9, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K

通过改变监管参数来建立可调整的基因逻辑门,具有多功能动态性能.

Tian Jiang1, Yuxi Teng1, Chenyi Li1

  • 1School of Chemical, Materials, and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.

ACS synthetic biology
|November 30, 2023
PubMed
概括

研究人员为合成生物学开发了新的遗传逻辑门. 这些可调节的门,包括缓冲器 (BUF),AND和NOT类型,可用于先进的代谢工程应用中精确控制基因表达.

关键词:
和 和 和 和 和 和 和 和.这就是BUF BUF.没有,没有,没有,没有.生物传感器生物传感器逻辑大门的逻辑大门.在p-coumaric 酸中.

更多相关视频

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K

相关实验视频

Last Updated: Jul 9, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K

科学领域:

  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.
  • 遗传电路设计的设计

背景情况:

  • 遗传逻辑门对于在合成生物学和代谢工程中调节基因表达至关重要.
  • 开发具有可适应动态性能的可调节门是扩大其应用的关键.
  • 现有的工具需要进一步改进,以构建复杂的遗传电路.

研究的目的:

  • 设计和描述新的遗传逻辑门,包括缓冲器 (BUF),AND和NOT门,以加强基因表达控制.
  • 为了证明这些门的实用性,使用p-酸生物传感器系统.
  • 使用开发的门来构建和评估双功能遗传电路.

主要方法:

  • 研究影响缓冲器 (BUF) 遗传逻辑门的参数,使用p-酸生物传感器.
  • 通过将生物传感器元素与TetR或LacI监管系统集成来构建和遗传逻辑门.
  • 通过将BUF门与反意义RNAs (asRNAs) 或单导 RNAs (sgRNAs) 结合,开发了p-coumaric acid触发的 NOT 门.

主要成果:

  • 成功设计和表征可调节的BUF,AND和NOT遗传逻辑门.
  • 证明了具有评估直角性的双功能遗传电路的构建.
  • 验证了p-酸生物传感器系统作为门开发的概念验证.

结论:

  • 开发的遗传逻辑门为精确的基因表达调节提供了多功能动态性能.
  • 这些门是促进代谢工程和合成生物学应用的宝贵工具.
  • 这项研究为构建更复杂和更复杂的遗传电路提供了基础.