用死亡益生菌涂层的抗菌表面的机制洞察力
Weixiong Zhang1, Lu Qian1, Bingen He1
1Faculty of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, P. R. China.
Langmuir : the ACS journal of surfaces and colloids
|November 30, 2023
概括
死亡的益生菌表面通过释放抗微生物溶解物来抑制Pseudomonas aeruginosa. 不活化方法控制释放,影响细菌粘附和生物膜形成.
科学领域:
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 像Lactobacillus rhamnosus GG (LGG) 这样的益生菌因其健康益处而闻名.
- 研究死益生菌的应用提供了新的抗菌策略.
- 了解细菌与修饰表面的相互作用对于开发新材料至关重要.
研究的目的:
- 为了探索表面的抗微生物作用功能化与非活化的Lactobacillus rhamnosus GG.
- 研究不同无活化方法对益生菌疗效的影响.
- 阐明死亡益生菌抑制Pseudomonas aeruginosa的机制.
主要方法:
- 用不活化的LGG装饰的表面的制造.
- 使用数字全息显微镜 (DHM) 和原子力显微镜 (AFM) 研究细菌的行为和粘附.
- 使用RNA测序和代谢分析来理解适应性反应.
主要成果:
- 死亡的LGG表面改变了Pseudomonas aeruginosa (PAO1) 的游泳行为,减少了化学反应和增加了鞭毛组装.
- 与对照组相比,在死LGG表面上观察到PAO1粘附和生物膜形成的显著减少.
- 细菌的适应性反应包括关键信号通路的下调,例如Pseudomonas quinolone信号.
结论:
- 抗菌活性归因于从死亡的LGG中逐渐释放抑制溶解物.
- 溶解酸释放的速度和顺序取决于细胞膜的完整性,受无活化方法的影响.
- 死的益生菌功能化的表面是控制细菌生长和生物膜形成的有希望的方法.
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