标志着APOBEC编辑,净化选择,框架转移和框架内无意义突变在块性皮肤病病毒的微演变中的迹象
Perumal Arumugam Desingu1, T P Rubeni1, K Nagarajan2,3
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru, India.
Frontiers in microbiology
|November 30, 2023
概括
型皮肤病病毒 (LSDV) 的进化是由基因变异,如移和无意义突变所驱动的. 净化选择,可能受到APOBEC编辑的影响,在LSDV中形成了codon使用偏差.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子进化分子进化
- 基因组学就是基因组学.
背景情况:
- 结节性皮肤病病毒 (LSDV) 在反动物中造成重大经济损失,并从非洲蔓延到全球.
- 驱动LSDV爆发的进化机制,包括选择压力和遗传变异,仍然不太清楚.
- 以前的研究还没有完全阐明子选择,APOBEC编辑和特定突变在LSDV进化的作用.
研究的目的:
- 调查LSDV.中的进化驱动因素的作用,例如编码子选择和APOBEC编辑.
- 识别和表征LSDV菌株内的遗传变异,包括框架转移和框架内无意义突变.
- 了解微观进化模式,并预测LSDV的未来进化轨迹.
主要方法:
- 分析各种全球疫情中LSDV菌株的完整基因组序列.
- 在特定的LSDV基因中识别和分类遗传变异,包括框架转移和框架内无意义突变.
- 评估选择压力对编码子使用偏差和APOBEC编辑的潜在作用.
主要成果:
- 在LSDV035,LSDV019,LSDV134和LSDV144基因中发现了框架转移突变.
- 在LSDV026,LSDV086,LSDV087,LSDV114,LSDV130,LSDV131,LSDV145,LSDV154,LSDV155,LSDV057和LSDV081基因中发现了无意义的内部突变.
- 野生类型的LSDV集群显示出不同的地理分布,重组病毒主要分布在亚洲和俄罗斯. 来自特定国家的病毒表现出独特的突变特征.
结论:
- 净化选择,可能是由APOBEC编辑驱动的,是影响LSDV中代码使用偏差的主要力量.
- 特定的框架转移和框架内无意义突变区分了在不同国家传播的LSDV菌株.
- 了解LSDV微演变对于改善流行病学诊断,疫苗开发和预测未来疫情至关重要.
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