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开发一种抗微生物耐药性等离子体转移基因数据库,用于肠道细菌

Suad Algarni^1,2, Steven L Foley¹, Hailin Tang³

¹Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR, United States.

Frontiers in bioinformatics

|November 30, 2023

概括

抗微生物耐药性 (AMR) 基因通过细菌结合通过塑传播. 一个新的数据库和工具分析了这些等离子体转移基因,有助于理解AMR传播.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
生物信息学是一种生物信息学.

背景情况:

第四类分泌系统 (T4SSs) 对于细菌的结合至关重要,它们通常被编码在携带抗菌素耐药性 (AMR) 基因的质粒上.
这些抗药性质粒的转移加速了细菌群体内的AMR传播.

研究的目的:

开发一个全面的数据集和计算工具来分析与AMR相关的等离子体转移基因.
评估这些转移基因在不同类型的等离子体中的遗传多样性和功能作用.

主要方法:

在GenBank.中从AMR等离子体中识别并提取转移基因.
创建了等离子体转移基因数据集,并开发了分析工具 (等离子体转移因子概况评估和等离子体转移因子比较).
使用等离子体和全基因组测序数据验证了数据库和工具.

主要成果:

开发的等离子体转移基因数据库和分析工具对于评估等离子体类型及其与T4SSs的关联是有效的.
这些工具有助于人们更好地了解结合性质粒是如何促进抗菌素耐药性基因传播的.
序列多样性分析显示了不同类型的等离子体中关键转移基因和ATPase同类基因的变异.

结论:

新的数据库和工具为研究等离子体介导的AMR传播提供了宝贵的资源.
对等离子体转移基因的计算分析对于跟踪和理解抗菌素耐药性的传播至关重要.

关键词:

它们的属性是 Enterobacteriaceae.世界地质系统分析工具 WGS分析工具结合方式结合方式我们的数据库数据库数据库数据库.塑料体中的塑料.转移基因转移基因转移基因是什么

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