早期诊断急性损伤的生物标志物,在老鼠模型中使用西斯胺诱导的毒性
Sahadeb Jana1,2, Palash Mitra2, Ananya Dutta1,2
1Biodiversity and Environmental Studies Research Center, Midnapore City College, Kuturiya, Bhadutala, Midnapore, Paschim Medinipur, Pin- 721129, West Bengal, India.
Current research in toxicology
|November 30, 2023
概括
早期发现急性损伤 (AKI) 是至关重要的. 像KIM-1和NGAL这样的新生物标志物显示出在传统标志物如BUN和血清胆固醇升之前识别损伤的潜力.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 生物标志物发现发现
- 毒理学 毒理学 毒理学
背景情况:
- 急性损伤 (AKI) 可以导致慢性病 (CKD) 和严重的并发症.
- 目前用于AKI检测的方法缺乏实时灵敏度和准确性.
- 需要早期,可靠的生物标志物来预防末期病.
研究的目的:
- 开发一种特定而敏感的模型,用于早期识别雄性白化老鼠中西斯胺诱导的损伤.
- 用多个生理学,生化学,基因组学和基因病理学标记来比较依赖持续时间的毒性影响.
- 在传统标志物 (BUN,sCr) 保持正常的情况下调查功能障碍的发病情况.
主要方法:
- 给雄性白色大鼠注射一次腹膜内西斯丁剂 (10毫克/公斤).
- 在注射后的1,2,3,5,7天收集了血,尿液和脏组织样本.
- 评估了生理,生化,基因组 (mRNA表达) 和基因病理标志物,包括KIM-1,IL-18,尼弗林,NGAL和血清囊素C.
主要成果:
- 尿液KIM-1,IL-18,NGAL和血清CysC水平在第3天显著增加,而BUN和sCr仍然正常.
- 在脏组织中,KIM-1,IL-18,Cys C和NGAL的升调mRNA表达和降调nephrin表达表明早期毒性.
- 西方斑点证实了脏组织中KIM-1,IL-18和NGAL的上调蛋白表达.
结论:
- 一系列功能障碍指标,包括KIM-1,IL-18,NGAL和Cys C,与目前的标志物相比,可提供更早,更可靠的AKI诊断.
- 这些新型生物标志物可以在非常早期的阶段检测损伤,甚至在BUN和sCr的显著变化之前.
- 这种方法提供了一种更有效的策略来管理AKI并防止进展到CKD.
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