在Archaeplastida中对VPS13蛋白的遗传学和结构分析揭示了它们在Viridiplantae中的复杂进化历史
Sébastien Leterme1, Olivier Bastien1, Riccardo Aiese Cigliano2
1Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG, LPCV, Grenoble, France.
Contact (Thousand Oaks (Ventura County, Calif.))
|November 30, 2023
概括
脂质转移蛋白家族VPS13在光合作用生物中具有复杂的进化历史. 这项研究揭示了新的家族和基因重复,提供了关于它们在植物中的功能的见解.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 植物科学 植物科学
背景情况:
- VPS13蛋白质是重要的脂质转移蛋白质,参与了细胞膜动力学和细胞器生物生成.
- 虽然在酵母和哺乳动物中得到了很好的研究,但VPS13在光合作用生物 (Archaeplastida) 中的进化和功能在很大程度上仍未被探索.
- 阿拉比多普西斯塔利亚纳具有四种VPS13类似物 (AtVPS13S,X,M1,M2),它们在植物发育中起着已知的作用.
研究的目的:
- 调查VPS13蛋白质在Archaeplastida中的进化历史和结构组织.
- 识别和描述光合作用系中的新型VPS13家族和对应物.
- 了解植物中VPS13进化的多样化和潜在的功能影响.
主要方法:
- 在Archaeplastida中对VPS13蛋白序列的家族遗传学分析.
- 域分析以确定保存和新型蛋白质架构.
- 结构建模用于预测蛋白质折叠和功能域.
- 比较基因组学来追踪基因复制和重排事件.
主要成果:
- 在某些Archaeplastida群体中确认了人类VPS13B的正确性.
- 发现了两个新的VPS13家族:VPS13R (Rhodophytes) 和VPS13Y (Chlorophytes,Streptophytes). 这两个新的VPS13家族是:VPS13R (Rhodophytes) 和VPS13Y (Chlorophytes,Streptophytes).
- 在Viridiplantae中阐明复杂的进化途径,包括基因增加,重复和域重排.
- 在不同的植物谱系中识别了不同的相对数,其中一些Charophytes拥有多达六个VPS13相对数.
- 突出了VPS13M和VPS13X类似器的特定结构特征.
结论:
- VPS13家族在光合作用生物体中表现出复杂的进化动态.
- 识别新的VPS13家族和类比物为未来的功能研究提供了基础.
- 了解VPS13的进化对于破译它们在植物生命基本过程中的作用至关重要.
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