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Reem Sabry¹, Diana R May¹, Laura A Favetta¹

¹Reproductive Health and Biotechnology Laboratory, Department of Biomedical Sciences Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.

Frontiers in cell and developmental biology

|November 30, 2023

概括

微RNA-21 (miR-21) 在调节牛卵细胞和粒粉细胞中的DNA甲基化方面发挥着关键作用. 抑制miR-21对DNA甲基化酶产生影响,这表明它参与了双A.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
毒理学毒理学毒理学

背景情况:

微RNA-21 (miR-21) 对于卵细胞和颗粒细胞的功能至关重要,影响着细胞亡和表观遗传调节.
在雌性性生殖过程中,DNA甲基化是至关重要的,并且可以通过microRNAs进行调节.
双A (BPA) 干扰卵细胞成熟和粒粉细胞功能,可能通过涉及DNA甲基化和微RNA的表观遗传途径.

研究的目的:

为了研究miR-21在调节牛体积卵细胞复合体 (COCs) 和粒粉细胞内的DNA甲基化中的作用.
探索双甲 (BPA) 暴露对miR-21介导的DNA甲基化的影响.
阐明miR-21,DNA甲基化酶和BPA对雌性性子细胞的影响之间的关系.

主要方法:

使用抗miR-21锁定核酸 (LNA) 来抑制牛的COC和粒粉细胞中的miR-21活性.
评估了5-甲基细胞因子 (5mC) 和5-基甲基细胞因子 (5hmC) 的水平.
使用qPCR和西式涂抹对DNA甲基化和脱甲基化酶的量化基因表达.

主要成果:

暴露于BPA降低了粒粉细胞中的5mC水平,与降低DNMT1/DNMT3A和增加TET2.2相关.
抑制miR-21导致DNMT1,DNMT3A和TET2的蛋白质水平在COC和颗粒细胞中增加.
在miR-21信号传递,DNA甲基化和BPA暴露效应之间观察到显著的相关性.

结论:

miR-21是牛的COC和粒状细胞中DNA甲基化的一个关键调节剂.
miR-21抑制会影响关键DNA甲基化和脱甲基化酶的表达.
这些发现突出了miR-21在调解BPA对女性生殖细胞的表观遗传影响中的作用.

关键词:

通过DNA甲基化.双 A 是一种双 A.颗粒状细胞是颗粒状细胞.据报道,米R-21的发射器为米R-21 卵子细胞卵子细胞

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