正规的单链DNA结合蛋白不是一个重要的复制因子,而是Saccharolobus islandicus中的RNA伴侣
Yuanxi Xiao1, Zhichao Jiang1, Mengqi Zhang1
1CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao 266237, China.
iScience
|November 30, 2023
概括
古老的单链DNA结合蛋白 (SSBs) 不对细胞活力至关重要. 这些蛋白质,像SisSSB一样,溶解dRNA和DNA/RNA杂交体,这种作用在Crenarchaeota中得到保护.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 考古研究 考古研究
背景情况:
- 单链DNA结合蛋白 (SSB) 传统上被认为对DNA复制至关重要.
- 在*Saccharolobus islandicus*中,正规 (SisSSB) 和非正规 (SisDBP) SSB 的作用尚未被探索.
研究的目的:
- 调查SisSSB和SisDBP对S.islandicus*细胞活力的关键性.
- 阐明考古经典SSB,特别是SisSSB的功能性作用.
主要方法:
- 在S.islandicus*中进行基因删除实验 (Δ*Sisssb*, Δ*Sisssb*Δ*Sisdbp*).
- 在体外测试以评估SisSSB在dsRNA和DNA/RNA杂交物上的化活性.
- 在大肠杆菌*中进行功能补充测定.
主要成果:
- SisSSB和SisDBP对于S.islandicus的生存能力并不是必不可少的.
- 在较低温度 (55°C) 上观察到删除突变的增长减少,与SisSSB水平相关.
- SisSSB在dSRNA和DNA/RNA杂交物以及大肠杆菌中的RNA发针上表现出体外融活性.
- 核心SisSSB域可以补充大肠杆菌中缺乏冷震蛋白的缺失.
- 这些活动保存在Crenarchaeota缺乏细菌Csp同类的正规SSB中.
结论:
- 考古经典的SSB,就像SisSSB一样,不是主要的DNA处理因素.
- 在涉及双链RNA或DNA/RNA混合物的化过程中,SisSSB起着至关重要的作用.
- 在dsRNA/混合融中,古老的SSBs的功能作用是保留的,并且独立于细菌冷冲击蛋白质同类物.
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