缺氧内皮细胞衍生的外体细胞通过改善内皮细胞功能和促进M2巨细胞的两极分化来促进糖尿病伤口愈合
Peng Cheng1,2, Xudong Xie1,2, Liangcong Hu1,2
1Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Bioactive materials
|November 30, 2023
概括
富含lncHAR1B的低毒外体,通过改善内皮细胞功能和巨细胞两极分化,加速糖尿病伤口愈合. 这项研究引入了一种新的治疗策略,使用外体载荷的水凝来有效治疗糖尿病伤口.
科学领域:
- 再生医学是一种再生医学.
- 生物技术是生物技术.
- 分子生物学分子生物学
背景情况:
- 耐火性糖尿病伤口缺乏有效的治疗方法.
- 缺氧外体显示出促进糖尿病伤口愈合的潜力.
- 具体机制需要进一步研究.
研究的目的:
- 研究缺氧外体在增强糖尿病伤口愈合方面的疗效.
- 为了阐明这种过程背后的分子机制.
- 为创伤愈合开发一个实用的外体输送平台.
主要方法:
- 高通量测序以识别缺氧外基因组中差异表达的lncRNA.
- 在高葡萄糖条件下对内皮细胞和巨细胞的体外研究.
- 在体内实验中,在糖尿病伤口模型中使用缺氧外体载荷的水凝.
主要成果:
- 低毒性外体细胞与正常性外体细胞相比,显著提高了 lncHAR1B 的调节.
- lncHAR1B促进了内皮细胞功能和M1到M2巨细胞的两极分化.
- lncHAR1B与BHLHE23直接相互作用,促进KLF4的表达.
- 外体载荷的水凝在体内加速伤口愈合.
结论:
- 缺氧外体,特别是lncHAR1B,为糖尿病伤口愈合提供了一个有前途的治疗策略.
- 这项研究阐明了一种涉及IncHAR1B,BHLHE23和KLF4.4的新型分子途径.
- 带有外体荷载的水凝是加速伤口修复的可行交付平台.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
01:19Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K