Pulsatillae radix提取物通过调节肠道微生物群和小鼠的炎症信号通路来缓解DSS诱导的大肠炎
Xianping Li1,2, Zhihuan Wang1, Hongyuan Gao3
1State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018RU010), Chinese Academy of Medical Sciences, Beijing, 102206, China.
Heliyon
|November 30, 2023
概括
豆的成分Pulsatillae radix通过恢复肠道微生物群平衡和调节炎症通路,有效地治疗小鼠的性结肠炎. 这项研究强调了其在炎症性肠病中的治疗潜力.
科学领域:
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠道疾病,对全球健康影响越来越大.
- 传统中医药,像Baitouweng (BD),在治疗UC具有历史性的使用,但其机制仍然不清楚.
- Pulsatillae radix是BD的关键成分,需要对其对大肠炎的特定治疗作用进行研究.
研究的目的:
- 评估Pulsatillae radix在DSS诱导的性结肠炎的小鼠模型中的治疗疗效.
- 阐明Pulsatillae radix改善结肠炎的潜在机制,重点关注肠道微生物群和炎症途径.
主要方法:
- 超高性能液体色谱 (UPLC) 用于识别Pulsatilla chinensis (Pulsatillae radix) 中的活性化合物.
- 16S rRNA测序分析了大肠炎模型小鼠的阴中的肠道微生物群组成.
- 转录组测序评估了结肠组织中的基因表达特征.
主要成果:
- Pulsatillae radix显著改善了DSS诱导的大肠炎的临床症状,降低了疾病活动指数 (DAI) 和结肠缩短.
- 用Pulsatillae radix进行的治疗逆转了肠道微生物群的失生症,改变了关键细菌类和属的丰富性.
- 转录组分析显示,Pulsatillae radix调节基因表达,调高NOD类受体信号通路,调低细胞因子-细胞因子受体相互作用,TNF和IL-17信号通路.
结论:
- 在小鼠模型中,Pulsatillae radix在缓解DSS诱导的性结肠炎方面表现出显著的治疗效果.
- 该机制涉及肠道微生物组合的调节和关键炎症信号通路的调节.
- 这些发现支持使用Pulsatillae radix作为性结肠炎的潜在治疗剂.
相关概念视频
01:17Drugs for Treatment of Diarrhea-Predominant IBS
182
Diarrhea-predominant irritable bowel syndrome (IBS-D) is a subtype of IBS characterized primarily by frequent, loose, or watery stools, abdominal pain, and abdominal discomfort. Therapeutic approaches to managing IBS-D include dietary changes, stress management techniques, and pharmaceutical interventions.
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
Two specific drugs used in the treatment are alosetron (Lotronex) and eluxadoline (Viberzi). Alosetron, a 5-HT3 antagonist, works by slowing the movement of stools in the gut, reducing bowel...
182
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
335
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
335
01:29Drugs for Treatment of Ulcerative Colitis in IBD
145
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
145