LncBIRC3-OT通过与RELA相互作用来促进质瘤的恶性进展,以调节斯坦尼奥卡尔-1表达的上升
Renjie Wang1,2, Qi Li1, Xiaolei Chu1
1Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China.
Heliyon
|November 30, 2023
概括
长非编码RNA BIRC3-OT (lncBIRC3-OT) 通过启动stanniocalcin-1转录来促进质瘤的进展. 低调 lncBIRC3-OT 抑制了结质瘤细胞的生长,迁移和入侵,这表明它是潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 质瘤是最常见的恶性脑瘤,占内瘤的80%.
- 长非编码RNAs (lncRNAs) 越来越多地被认可为它们在瘤发展中的作用,包括质瘤.
- lncRNAs影响瘤生长,血管新生,转移和治疗耐药性.
研究的目的:
- 为了研究lncBIRC3-OT (NONHSAT159592.1) 在质瘤中的作用.
- 确定IncBIRC3-OT表达对质瘤细胞行为的功能影响.
- 为了阐明IncBIRC3-OT促进质瘤进展的分子机制.
主要方法:
- RNA测序 (RNA-seq) 用于选高表达的 lncRNAs 在质瘤中.
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 验证了 lncBIRC3-OT表达.
- lncBIRC3-OT在U87和U251质瘤细胞系中被击败,以评估功能影响.
主要成果:
- 发现 lncBIRC3-OT 在质瘤组织中表达很高.
- 低调 lncBIRC3-OT 显著抑制了结质瘤细胞的增殖,殖民地形成,迁移和入侵.
- 从机理上讲,IncBIRC3-OT被证明可以将RELA蛋白招募到斯坦尼奥卡尔辛-1 (STC1) 促进剂中,从而驱动STC1转录.
结论:
- lncBIRC3-OT作为促进质瘤进展的关键调节剂.
- lncBIRC3-OT/RELA/STC1轴是推动质瘤发展的关键途径.
- lncBIRC3-OT代表了质瘤治疗的潜在治疗标.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
The Nucleolus
8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K
The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K


