遗传学和蛋白质结构分析提供了对CD36域"Apex"在Eukarya中Scavenger受体B类蛋白中的进化和多样化的洞察
Reed T Boohar1, Lauren E Vandepas1, Nikki Traylor-Knowles2
1Department of Biology, University of Miami, Coral Gables, Florida, USA.
Genome biology and evolution
|November 30, 2023
概括
清理器受体B (SR-B) 蛋白质,包括CD36域,是古老的,在真核生物中发现. 它们的进化涉及CD36ectodomain的序列和结构变化,影响联体感应特异性.
科学领域:
- 进化生物学是进化的生物学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 由CD36ectodomain特征的B类拾尸体受体 (SR-Bs) 对于双边人的先天免疫和代谢过程至关重要.
- 以前的研究表明SR-Bs的起源很古老,但它们在Eukarya中的多样性仍然未被充分探索.
研究的目的:
- 描述 SR-B 同类物种在整个真核生物中的进化多样性.
- 在真核生物进化过程中调查CD36外域内的结构性保护和变异.
主要方法:
- 来自165种不同的真核生物基因组和转录组的SR-B同类生物信息分析.
- 对保存的氨基酸基因和蛋白质三级结构进行比较分析,重点是CD36ectodomain.
主要成果:
- 在主要的真核超群中存在SR-B同类物,这表明它们存在于最后一个真核共同祖先中.
- 在CD36ectodomain中保留了一个非对称的β桶三级结构,在整个Eukarya中保持着.
- 在CD36ectodomain的顶部区域中确定了独立的,特定于谱系的序列扩张,这对于连接体传感至关重要.
结论:
- CD36外主体及其相关的SR-B蛋白质是真核生物学的古老,保存的特征.
- 进化变异,特别是在CD36顶部区域,可能有助于在不同的真核细胞系中观察到的多样化的连接体感应特异性.
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