来自Nrxn3的轨道前额内圆形RNA介导奖励学习和奖励动机
Konrad R Dabrowski1, Gabriele Floris2, Aria Gillespie2
1Center for Substance Abuse Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA; Department of Biology, Temple University, Philadelphia, PA, USA.
Progress in neurobiology
|November 30, 2023
概括
轨道前皮层 (OFC) 中的循环RNA (circRNAs) 最近与寻求奖励有关. 循环Nrxn3的下调增强了糖糖的动机,揭示了奖励行为的新型分子机制.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 轨道前皮层 (OFC) 对于奖励处理和动机行为至关重要.
- 治理OFC中介的奖励寻求的分子机制尚未完全理解.
- 循环RNAs (circRNAs) 在奖励回路中的作用在很大程度上仍未被探索.
研究的目的:
- 为了在OFC中对食欲奖励的反应中对circRNAs进行分析.
- 研究circNrxn3在调节寻求奖励行为中的功能.
- 在OFC中识别circNrxn3的分子相互作用.
主要方法:
- 糖在动物中的自我管理范式.
- 用于circRNA分析的RNA测序和定量PCR (qPCR).
- 在体内敲除circNrxn3和随后的转录组分析.
- RNA-免疫沉降 (RIP) 试验.
主要成果:
- 确定了92个OFC环RNAs,这些环RNAs受糖摄入量调节.
- 观察到circNrxn3的下调,circRNA是由Nrxn3.3衍生而来的.
- 与学习/记忆和Nrxn3拼接相关的circNrxn3 knockdown改变基因表达.
- circNrxn3 knockdown增加了糖的自我管理和动机.
- 已经证明circNrxn3与拼接因子SAM68.8结合.
结论:
- circNrxn3是第一个确定调节奖励行为的circRNA.
- circNrxn3与SAM68相互作用,影响RNA处理和基因表达.
- 这项研究揭示了OFC驱动的奖励寻求中的新型分子途径.
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