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DNase I 拯救了被中性粒细胞外细胞陷所困住的山羊精子

Zhengkai Wei¹, Hongrong Hong², Wei Liu²

¹College of Veterinary Medicine, Southwest University, Chongqing, 400715, PR China; College of Life Sciences and Engineering, Foshan University, Foshan, 528225, Guangdong Province, PR China.

Developmental and comparative immunology

|November 30, 2023

概括

精子可以在山羊中触发中性细胞外细胞陷 (NETs),损害精子. 脱氧核糖核酶I (DNase I) 降解这些NET,保护精子质量和运动性,为生殖免疫提供了新的见解.

科学领域:

生殖免疫学生殖免疫学
羊的养殖羊的养殖
细胞生物学细胞生物学

背景情况:

在山羊人工授精可以导致精子诱导的炎症.
中性粒细胞释放中性粒细胞外细胞陷 (NETs),以捕获精子等外来实体.
脱氧核糖核酶I (DNase I) 可以降解NET,并已被用于改善精子质量.

研究的目的:

调查山羊中精子诱导的NET形成的机制.
探索DNase I对NET和精子质量的影响.

主要方法:

通过光学显微镜评估精子质量.
NET结构可视化使用免疫光染色.
与抑制剂和PicoGreen一起研究的NET形成机制.

主要成果:

精子诱导NET形成由糖解,NADPH氧化酶,ERK1/2和p38途径调节.
网络含有DNA,氨酸基因素H3 (citH3) 和弹性酶 (NE).
DNase I 降解了 NET,保持了精子等离子体膜的完整性和运动性.

结论:

精子诱导的NETs会损害精子,但DNase I通过降解NETs来抵消这种影响.
DNase I 保护山羊的精子质量和运动性.
这些发现为山羊的生殖免疫和潜在的治疗策略提供了新的见解.

关键词:

脱氧核糖核酸酶 I 的作用.羊的精子是羊的精子.中性粒细胞外细胞陷生殖免疫力生殖免疫力

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