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过高homocysteinemia可能会加剧腹腔大动脉动脉瘤的形成通过上调RASSF2加剧调节

Zongwei Liu¹, Guilin Feng¹, Yonghui Chen¹

¹Department of Vascular surgery of Tianjin Medical University General Hospital, Tianjin, PR China.

|November 30, 2023

概括

超同胞蛋白血症 (HHcy) 通过增加Ras关联域家族成员2 (RASSF2) 和大动脉内皮细胞中的介质素-1β (IL-1β) 来加剧腹腔大动脉动脉瘤 (AAA). 这些发现表明AAA预防和治疗的新治疗点.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

腹腔大动脉瘤 (AAA) 是一种危及生命的疾病,死亡率高.
目前对AAA的治疗方法有限,没有药物有效降低其风险.
超同胞蛋白血症 (HHcy) 与AAA发病率的增加有关,但其分子机制尚不清楚.

研究的目的:

研究通过HHcy加剧AAA形成的分子标.
整合转录组数据和实验验证,以确定关键分子参与者.

主要方法:

与AAA相关的公共微阵列和单细胞RNA测序数据集的综合分析.
生物信息学分析以识别HHcy相关AAA.中的差异表达基因.
在人类AAA组织中使用逆转录定量聚合酶链反应 (RT-qPCR) 和免疫光学验证基因表达.

主要成果:

转录组分析确定了Ras关联域家族成员2 (RASSF2) 和互白素-1β (IL-1β) 作为HHcy介导AAA中的潜在关键基因.
建议的单细胞RNA-seq RASSF2 损害了内皮细胞功能,并促进了AAA中的炎症透.
与对照组相比,RT-qPCR和免疫光检测证实AAA组织中的RASSF2和IL-1βmRNA和蛋白质水平明显上调.

结论:

HHcy可能通过上调大动脉内皮细胞中的RASSF2和IL-1β表达来促进AAA的发展.
RASSF2,特别是在内皮细胞中,与AAA的病原发生有关.
这些发现突出了RASSF2和IL-1β作为管理HHcy相关AAA的潜在治疗点.

关键词:

腹腔大动脉动脉瘤腹腔大动脉动脉瘤超高同胞蛋白质血症的发生.拉斯协会域名家族成员2 2 一个单细胞RNAseqqq

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