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使用Agrobacterium tumefaciens介导的转化方法对Cochliobolus heterostrophus进行有效的向基因删除方法

Jiaying Sun¹, Rui Yang¹, Yujia Liu¹

¹College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, PR China.

Journal of microbiological methods

|November 30, 2023

概括

一种新的Agrobacterium tumefaciens介导转化 (ATMT) 方法有效地删除了Cochliobolus heterostrophus中的基因,这是一种主要的玉米病原体. 这种改进的技术有助于了解真菌的毒性,并制定疾病控制策略.

科学领域:

植物病理学植物病理学
分子菌学分子菌学.
遗传学是一个遗传学.

背景情况:

科克里奥布洛斯 (Cochliobolus heterostrophus) 是南方玉米叶病原体,也是一个典型的死菌致病原体.
传统的基因淘汰方法,如原生质介导的转化,是低效和昂贵的.
局限性包括原始质细胞的准备,DNA产量,时间和费用等问题.

研究的目的:

建立和优化C. heterostrophus.的高效基因删除方法.
为了利用Agrobacterium tumefaciens介导的转化 (ATMT) 进行基因操纵.
研究C. heterostrophus基因的功能及其在毒性中的作用.

主要方法:

开发和优化一个Agrobacterium tumefaciens介导的转化 (ATMT) 协议.
转换效率评估为每10^5 conidia的85-88个变压器.
同类重组效率确定为大约68.3%.

主要成果:

使用ATMT方法成功生成了C. heterostrophus的六种基因淘汰突变.
与野生型菌株相比,突变菌株表现出改变的表型.
淘汰突变体的毒性显著减少.

结论:

已建立的ATMT系统为C. heterostrophus的基因操纵提供了一个高效的工具.
这种方法有助于更好地了解基因功能及其与真菌毒性的关系.
开发的系统可以帮助开发新的策略来管理南方玉米叶病.

关键词:

这是一种由Agrobacterium tumefaciens介导的转化.科克里奥布洛斯 (Cochliobolus) 是一种异构的植物.目标基因的删除目标基因的删除

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