转录因子BZR2/MYC2在梨休眠期间调节了布拉西诺固醇和斯酸交叉
Xuxu Wang1,2,3,4, Jia Wei2,3,4, Jiahao Wu1,2,3,4
1Hainan Institute of Zhejiang University, Sanya, Hainan 572000, PR China.
Plant physiology
|November 30, 2023
概括
铜类固醇和莉酸通过调节BZR2/MYC2通路来促进梨芽的破裂. 这一途径影响着吉伯雷林合成和休眠相关基因表达,对于克服梨的冬季休眠至关重要.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 园艺科学 园艺科学
背景情况:
- 芽休眠是植物的一个关键的过冬策略,由酸和贝林等荷尔蒙信号调节.
- 虽然已知ABA和GA的作用,但其他植物激素对芽休眠释放的影响,特别是在梨 (Pyrus pyrifolia) 中,仍未得到充分探索.
- 了解这些荷尔蒙相互作用是管理芽破裂和改善果实生产的关键.
研究的目的:
- 为了研究类固醇 (BRs) 和酸 (JA) 在调节梨芽休眠释放中的作用.
- 阐明BR和JA作用背后的分子机制,重点关注关键的转录因子和基因表达.
- 探索BR和JA信号通路之间的交叉声在控制休眠释放中的作用.
主要方法:
- 外源性24-epibrassinolide (EBR) 和甲基斯蒙酸 (MeJA) 的应用,以评估它们对梨芽破裂的影响.
- 对基因表达的分析,包括DORMANCY-ASSOCIATED MADS-box (DAM) 基因和Gibberellin 20-氧化酶 (GA20OX) 基因.
- 通过过度表达和相互作用研究,研究了BRASSINAZOLE RESISTANT 2 (BZR2) 和MYELOCYTOMATOSIS 2 (MYC2) 的功能.
- 基因操纵和激素治疗后内源性激素水平 (GA,JA,JA-Ile) 的量化.
主要成果:
- 无论是EBR还是MeJA治疗都促进了梨芽的破裂,特别是在冷却不足的情况下,这表明它在休眠释放中发挥了积极作用.
- 过度表达PpyBZR2抑制了PpyDAM3的表达,并增加了内源性GA和JA水平,加速了芽裂.
- PpyBZR2与PpyMYC2直接相互作用,增强了PpyGA20OX1L1和PpyGA20OX2L2的转录,从而增加了GA3含量和更快的芽断裂.
- 观察到MeJA的双重效应:低度通过上调PpyGA20OX和GA3 / 4促进了芽裂,而高度通过减少PpyGA20OX激活和PpyBZR2对PpyDAM3的影响来抑制它.
结论:
- 铜类固醇和莉酸积极调节梨芽休眠释放.
- BZR2/MYC2介导的途径是BR和JA信号的核心,通过GA生物合成促进休眠释放.
- 这项研究揭示了BR和JA途径在控制梨芽破裂方面存在显著交叉,为园艺应用提供了新的目标.
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