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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Updated: Jul 9, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

解决复杂的纳米结构与 ptychographic 原子电子断层扫描.

Philipp M Pelz^1,2,3, Sinéad M Griffin^4,5, Scott Stonemeyer^5,6,7,8

¹Institute of Micro- and Nanostructure Research (IMN) & Center for Nanoanalysis and Electron Microscopy (CENEM), Friedrich Alexander-Universität Erlangen-Nürnberg, IZNF, 91058, Erlangen, Germany. philipp.pelz@fau.de.

Nature communications

|November 30, 2023

概括

此摘要是机器生成的。

图形电子断层扫描可以重建纳米材料中的原子结构. 这种新方法揭示了碳纳米管内以前未知的ZrTe2阶段,推进了材料科学和结构生物学.

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相关实验视频

Last Updated: Jul 9, 2025

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

科学领域:

材料科学材料科学材料科学
结构生物学结构生物学
纳米技术纳米技术

背景情况:

传输电子显微镜 (TEM) 对于原子规模的结构确定至关重要.
目前的方法如相对比TEM和原子电子断层扫描 (AET) 对具有光元素的异质纳米材料有局限性.

研究的目的:

开发和应用一种用于原子分辨率成像的新型图形电子断层扫描技术.
确定含有轻元素的复杂纳米材料的三维原子结构.

主要方法:

采集了3450万个使用电图电子断层扫描的衍射图案.
重建一个双壁碳纳米管 (DW-CNT) 的原子分辨率倾斜系列.
原子峰的类平均和亚像素定位.

主要成果:

成功重建了一个DW-CNT封装ZrTe三明治结构的原子结构.
识别了一个Zr11Te50结构,其中包含一个新的ZrTe2阶段在核心.
对光束敏感纳米材料进行原子分辨率成像的演示.

结论:

图形电子断层扫描使得复杂,异质纳米材料的原子分辨率结构确定成为可能.
这项研究揭示了一个新的ZrTe2阶段,扩大了对 telluride 系统的知识.
这种技术为分析各种科学领域的轻元素纳米材料开辟了新的途径.