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Updated: Jul 9, 2025

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绘制和建模人类结直肠癌与瘤微环境的相互作用

Ning Li^1,2, Qin Zhu^3,4, Yuhua Tian⁵

¹Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. Ningli@upenn.edu.

Nature communications

|November 30, 2023

概括

这项研究使用器官模拟结直肠癌 (CRC) 和瘤微环境 (TME) 相互作用. 人类巨细胞在瘤体中采用免疫抑制程序,揭示了对癌症进展的新见解.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
系统生物学系统生物学

背景情况:

瘤微环境 (TME) 极大地影响癌症的开始和进展.
在体内复杂性阻碍了对特定癌症-TME相互作用的理解.

研究的目的:

从单细胞转录组数据预测癌症-TME相互作用.
评估这些相互作用如何在人类瘤器官 (瘤) 培养物中发生变化.
为了在实验室中功能性地回顾骨髓细胞癌相互作用.

主要方法:

对人类结直肠癌 (CRC) 和小鼠CRC模型的单细胞转录基因映射.
在瘤培养物中的癌症-TME相互作用的分析.
在体外共同培养瘤与人类单细胞衍生的巨细胞.

主要成果:

瘤培养物减少与炎症和免疫细胞迁移相关的基因表达.
引入瘤体的人类巨细胞获得免疫抑制和亲瘤基因表达.
在巨细胞中观察到SPP1 (Osteopontin) 的升调,这是具有瘤效应的CD44联体.

结论:

瘤培养物为研究CRC-TME相互作用提供了一个缩小主义的平台.
在实验室中模拟骨髓瘤癌相互作用可以重复体内TME的关键方面.
这一框架有助于了解结直肠癌的进展,并开发有针对性的治疗方法.

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The Tumor Microenvironment

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