Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Flow Cytometry01:23

Flow Cytometry

13.0K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exploring the Potential Value of Inflammatory Response-Associated Genes in Chronic Rhinosinusitis with Nasal Polyps: An Integrated Bioinformatics and Experimental Validation Analysis.

Journal of inflammation research·2026
Same author

TCEP Induces Liver Injury Through Suppression of the PI3K/AKT Axis: Integrated Evidence from Epidemiology, Network Toxicology, and In Vivo Validation.

Chemico-biological interactions·2026
Same author

DAQplugin: Deep Learning based Real-time Model Evaluation Plugin for ChimeraX.

bioRxiv : the preprint server for biology·2026
Same author

Interfacial Redox Decoupling via Amphiphilic Carbon Dots for Highly Efficient Biphasic Photocatalytic H<sub>2</sub>O<sub>2</sub> Production and Selective Benzyl Alcohol Oxidation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Beta-Adrenergic Stimulation and <i>MYH7</i> G256E Mutant Gene Dosage Drive Hypertrophic Cardiomyopathy Phenotype Penetrance.

bioRxiv : the preprint server for biology·2026
Same author

Stress-Driven Accelerated Evolution and Ecological Network Reconfiguration in Extremophilic Microbial Communities.

Biology·2026
Same journal

Rewiring tumour mechanosensing to overcome CAR T cell resistance.

Nature biomedical engineering·2026
Same journal

Identifying and reprogramming softness-driven cancer stem-like cells overcomes CAR-T cell resistance in solid tumours.

Nature biomedical engineering·2026
Same journal

CD98hc-targeted antibody shuttles for central nervous system delivery with broad cross-species reactivity.

Nature biomedical engineering·2026
Same journal

AI-orchestrated design-build-test-learn is the future of mammalian biodesign.

Nature biomedical engineering·2026
Same journal

Lab-on-a-disc biosensing platform for folate level quantification.

Nature biomedical engineering·2026
Same journal

BoneCoT: multicentre validation of a whole-body skeleton foundation model for bone metastases guided by clinician-derived chain of thought.

Nature biomedical engineering·2026
查看所有相关文章

相关实验视频

Updated: Jul 9, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K

通过光谱编码的细胞条形码的高维多通道流细胞计.

Sheldon J J Kwok1, Sarah Forward2, Marissa D Fahlberg2

  • 1LASE Innovation Inc., Woburn, MA, USA. skwok@laseinno.com.

Nature biomedical engineering
|November 30, 2023
PubMed
概括
此摘要是机器生成的。

多通道高维流细胞计使用细胞条码来反复测量同一细胞随着时间的推移. 这种技术增强了用于免疫学和药物发现的单细胞分析能力.

更多相关视频

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
11:08

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

13.0K
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

21.3K

相关实验视频

Last Updated: Jul 9, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
11:08

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

13.0K
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

21.3K

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 生物技术是生物技术.

背景情况:

  • 流细胞计的进步对于免疫学,免疫瘤学,药物发现和疫苗开发至关重要.
  • 目前的局限性包括光体标记物中的光谱重叠和单一时间点测量.
  • 随着时间的推移,测量每细胞更多的蛋白质标记物是一个关键的挑战.

研究的目的:

  • 为了引入多通道高维流细胞计.
  • 为了使单个细胞的重复,高参数测量随着时间的推移.
  • 在复杂的生物研究中克服传统流细胞计的局限性.

主要方法:

  • 开发一种新的细胞条形码技术,使用发射近红外激光光的微粒.
  • 应用多通分析,其中单个细胞被测量了多次.
  • 使用活人外周血液单核细胞进行方法验证.

主要成果:

  • 在刺激前和刺激后证明了细胞的时间解析特征.
  • 使用10个标记面板实现分析,具有最小的光谱补偿.
  • 使用32标记面板在3个周期 (10-13标记/周期) 中实现了深度免疫类型.

结论:

  • 流式细胞计量中的细胞条码显著扩大了该技术的实用性.
  • 该方法促进了高维,多通道单细胞分析.
  • 这种方法简化了标记板设计,并减少了光谱溢出.