一种液体染色学-并联质谱检测试验,用于尿液中乙烯基葡萄化物和乙烯基硫酸盐的量化
Justin Dowis1, Xiang He2, Lori Pham1
1UCSF Health Clinical Laboratories, University of California San Francisco, San Francisco, CA, USA.
Methods in molecular biology (Clifton, N.J.)
|November 30, 2023
概括
乙基葡萄化物和乙基硫酸盐是检测乙醇消耗的理想尿液生物标志物,由于非侵入性采集和扩展的检测窗口. 一种经过验证的LC-MS/MS方法为临床使用提供了可靠的量化.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 乙糖化物 (EtG) 和乙硫酸盐 (EtS) 是乙醇消费的特定生物标志物.
- 与血液相比,尿液提供了一个非侵入性和方便的样本矩阵.
- 这些生物标志物为乙醇使用提供了比直接乙醇测量更长的检测窗口.
研究的目的:
- 开发和验证一种可靠的分析方法来量化人类尿液中的EtG和EtS.
- 建立一个可靠的检测方法来检测最近的乙醇消费.
主要方法:
- 液体染色学-双重质谱学 (LC-MS/MS) 使用负模式的电喷射电离.
- 选择反应监测 (SRM) 用于敏感和特定的检测.
- 简单的尿液稀释用于样品制备 (100μL),运行时间为7分钟.
主要成果:
- 临床验证的方法,报告范围为EtG的180100,000 ng/mL,EtS的5046,600 ng/mL.
- 两种生物标志物之间运行间不精确度<7%.
- 已证明适用于常规的临床和法医毒理学分析.
结论:
- 开发的LC-MS/MS方法对于检测尿液中的EtG和EtS是准确,精确和高效的.
- 这种测试为监测临床和法医环境中的乙醇使用提供了有价值的工具.
- 尿液EtG和EtS分析是评估最近酒精消耗的首选方法.
更多相关视频
11:06GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
17.0K
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
664
相关概念视频
Mass Spectrometry: Complex Analysis
792
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
792
Gas Chromatography–Mass Spectrometry (GC–MS)
4.3K
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
4.3K
