通过LC-MS/MS与同位素标记的内部标准对IOhexol血清清除的评估
Zhicheng Jin1, Roger L Bertholf2,3, Xin Yi4,5
1Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA.
Methods in molecular biology (Clifton, N.J.)
|November 30, 2023
概括
准确的功能评估对于活着的脏捐赠者至关重要. 一种新的液体染色学-双重质谱法 (LC-MS/MS) 方法准确地测量了iohexol清除率,以可靠地计算膜过率 (GFR).
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 分析化学 分析化学
背景情况:
- 精确测量膜过率 (GFR) 对于识别功能衰竭至关重要.
- 伊奥赫索尔是一种安全的,非放射性的,可用于GFR评估的外源性标记物.
- 现有的GFR测量方法在特定人群中可能有局限性,例如活着的脏捐赠者.
研究的目的:
- 描述一种经过验证的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于量化血清中的含量.
- 建立一个可靠的工作流程,以计算基于IOhexol清除的GFR.
- 评估该方法适用于评估活体脏捐赠者的功能.
主要方法:
- 对门诊患者进行IOHEXOL的管理.
- 在给药后采集三个定时血清样本.
- 血清蛋白沉,然后使用Thermo Vanquish UHPLC和TSQ Endura质谱仪进行LC-MS/MS分析.
主要成果:
- 开发的LC-MS/MS方法表现出强大的分析性能.
- 工作流程允许精确测量血清样本中的iohexol度.
- 总LC-MS/MS运行时间优化为2.5分钟.
结论:
- 描述的LC-MS/MS方法提供了一种可靠的方法,可以使用iohexol清除量来测量GFR.
- 这种工作流适用于评估看似健康的个体的功能,包括活着的脏捐赠者.
- 该方法支持准确的GFR确定,而不会损害脏捐赠者的健康.
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