通过高性能液体染色学-并联质谱法 (LC-MS/MS) 量化等离子体中的基酸
Aja Aravamudhan1, Cynthia L Johnson2, Jesse C Seegmiller3,4
1University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis, MN, USA.
Methods in molecular biology (Clifton, N.J.)
|November 30, 2023
概括
一种新的液体染色学-双重质谱法准确地测量了血中的酸 (MPA). 这种精确的治疗药物监测对于接受免疫抑制药物 (如mycophenolate mofetil) 的移植患者至关重要.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 临床化学 临床化学
背景情况:
- 菌酸莫菲蒂尔 (MMF) 和菌酸是固体器官移植后的重要免疫抑制剂.
- 由于高个体之间的变化和狭窄的治疗窗口,对酸 (MPA) 的治疗药物监测 (TDM) 是必不可少的.
- 在血中准确量化MPA对于优化患者的治疗结果具有临床意义.
研究的目的:
- 开发和验证一种快速,灵敏,特定和强大的LC-MS/MS方法,用于血中MPA量化.
- 建立一种可靠的分析工具,用于移植受体中MPA的TDM.
主要方法:
- 使用了一台Water's Xevo TQ-S微型质谱仪与Water's Acquity液态色谱系统.
- 采用正电喷射电离 (ESI) 和多重反应监测 (MRM) 来检测MPA.
- 菌酸碳氧乙烯 (MPAC) 作为内部标准.
主要成果:
- 该方法在0.25至40.00 mg/L的度范围内证明了线性.
- 获得了优异的测试内部不精确性 (2.7%在1.57 mg/L,3.9%在4.61 mg/L).
- 在20天内报告了强大的测试间不准确性 (4.0%在1.62 mg/L,5.6%在4.68 mg/L).
结论:
- 开发的LC-MS/MS方法提供了精确和准确的MPA在血中的量化.
- 这种经过验证的方法适用于移植患者中MPA的常规治疗药物监测.
- 该试验支持优化免疫抑制疗法和改善患者管理.
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