通过液体染色学-并联质谱法 (LC-MS/MS) 进行量化阿片类药物,阿片类药物和二甲的检测
Jacob Rininger1, Audrey Black1, Wenbo Li1
1Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH, USA.
Methods in molecular biology (Clifton, N.J.)
|November 30, 2023
概括
一种新的快速LC-MS/MS方法在4分钟内量化了尿液中的21种阿片类药物和二. 这种尿液药物测试在全国阿片类药物危机中支持临床决策.
科学领域:
- 临床化学 临床化学
- 法医毒理学 法医毒理学
- 分析化学 分析化学
背景情况:
- 国家阿片类药物危机是由合成阿片类药物驱动的,2021年有超过10万例过量死亡.
- 尿液药物检测对于临床决策和监测患者遵守受控物质至关重要.
- 随着药物趋势的发展,需要先进的实验室测试方法.
研究的目的:
- 描述一种快速液体色谱-并联质谱法 (LC-MS/MS) 方法.
- 在尿液中同时量化21种阿片类,阿片类和二类药物.
- 提供一种可靠的方法来评估患者的合规性和识别药物滥用.
主要方法:
- 开发了一种快速LC-MS/MS方法,用于同时量化.
- 该方法使用100μL的尿液和葡萄糖合物的酶性水解.
- 量化涉及多重反应监测 (MRM),使用七点校准曲线和化内部标准.
主要成果:
- 该方法实现了21种阿片类药物,阿片类药物和二胺类药物的同时定量.
- 分析时间不到4分钟.
- 该方法通过包括酶性水解来提供总药物测量.
结论:
- 这种快速的LC-MS/MS方法提供了一种强大而可靠的方法,用于测量尿液中的常见处方阿片类药物和二类药物.
- 该方法支持药物使用障碍患者的临床管理.
- 它通过提供及时和准确的药物检测结果,帮助解决国家危机.
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