液体染色学-双重质谱测量用于血清pentobarbital的定量
Hafiz Anas Yahya1, Patrick B Kyle2
1Department of Pathology, University of Mississippi Medical Center, Jackson, MS, USA.
Methods in molecular biology (Clifton, N.J.)
|November 30, 2023
概括
一种新的液体染色学-双重质谱法 (LC-MS/MS) 方法量化了人体血清中的巴比他. 这种快速测定对于管理需要镇静或诱导昏迷的创伤性脑损伤患者至关重要.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 神经科学是一个神经科学.
背景情况:
- 巴比他是一种短效巴比他酸,具有抗和镇静催眠的特性.
- 它在临床上用于镇静,控制,诱导昏迷和管理创伤性脑损伤 (TBI).
- 在TBI中,巴尔比他减少了大脑的血液流动和氧气消耗,降低了内压力并减轻了大脑缺血.
研究的目的:
- 开发和验证一种快速可靠的液体染色学-并联质谱法 (LC-MS/MS) 方法来定量巴比他.
- 为了解决缺乏商业上可用的多巴比塔尔量化方法的问题.
主要方法:
- 使用固体相提取 (SPE) 来从人血清中提取巴比他和D5-巴比他 (内部标准).
- 在负电离模式下使用LC-MS/MS与电喷射电离 (ESI) 进行分析.
- 通过多重反应监测 (MRM) 实现了量化和确认.
主要成果:
- 该方法证明了从0.5到100μg/ml的线性.
- 不精确度低于5%的变化系数.
- 托巴尔比塔尔在2.61分钟以7分钟的总运行时间化,只需要100μL的血清.
结论:
- 已经建立了一种灵敏,特定和快速的LC-MS/MS方法,用于在人血清中定量托巴比特.
- 这种方法适用于治疗药物监测,特别是在诸如TBI管理等重症监护机构.
- 低血清体积要求使其在临床使用中变得实用.
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