Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.3K
PCR01:32

PCR

207.1K
Overview
207.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Diagnostic pathways for lung cancer patients in Denmark: General practice events, first referral and stage at diagnosis.

Acta oncologica (Stockholm, Sweden)·2026
Same author

<i>'We don't use the word sustainability':</i> an interview study exploring general practitioner views on sustainability.

Scandinavian journal of primary health care·2026
Same author

Co-developing a vocational rehabilitation intervention for individuals with chronic low back pain across a regional spine centre and three municipalities in Denmark: a three-stage intervention development study guided by the Medical Research Council framework.

BMJ open·2026
Same author

Expert panel perspectives on improving the diagnosis and management of metabolic dysfunction-associated steatohepatitis in Denmark.

Scandinavian journal of gastroenterology·2026
Same author

Digital Health-Based Peer Support Ecosystem for Gestational Diabetes Mellitus in Vietnam (VALID II Study): Multistakeholder Cocreation and Pilot Study.

Journal of medical Internet research·2026
Same author

Videoconferences between remote-sitting specialist, patient and practice staff concerning low prevalent diseases and complex pathways in general practice clinics: a feasibility study.

Scandinavian journal of primary health care·2026

相关实验视频

Updated: Jul 9, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.7K

在一般实践中实施PCR测试 - 使用规范化过程理论进行定性研究.

Sinead Shahrzad1,2, Kirubakaran Balasubramaniam1, Marius Brostrøm Kousgaard2

  • 1Department of Public Health, University of Southern Denmark, Research Unit of General Practice, Odense, Denmark.

BMC health services research
|December 1, 2023
PubMed
概括

在一般实践中, Point-of-care 聚合酶链反应 (PCR) 测试显示没有常规使用的临床需要. 一般医生的工作量增加超过了非流行病环境中更快的结果的好处.

关键词:
评价 评价 评价实施 实施 实施规范化过程理论 规范化过程理论在POC中进行PCR测试.

更多相关视频

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
04:56

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Published on: May 16, 2025

318
Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

404.2K

相关实验视频

Last Updated: Jul 9, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

13.7K
Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
04:56

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Published on: May 16, 2025

318
Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
09:00

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies

Published on: May 22, 2012

404.2K

科学领域:

  • 传染病流行病学 传染病流行病学
  • 诊断技术评估 诊断技术评估
  • 一般实践 创新 创新

背景情况:

  • 随着COVID-19的流行,人们越来越需要快速,大规模的测试.
  • 基于社区的聚合酶链反应 (PCR) 测试面临着后勤和工作量方面的挑战.
  • 照顾点 (POC) PCR测试已成为病毒性呼吸道感染监测的潜在替代方案.

研究的目的:

  • 评估在一般实践中实施POC PCR测试的启用和抑制因素.
  • 评估POCPCR在急性呼吸道感染中的常规使用情况.
  • 了解一般医生对POC PCR技术的看法.

主要方法:

  • 在丹麦西兰东部的14个普通诊所进行POCPCR测试的6周试验.
  • 在测试期间之前和之后,与全科医生 (GP) 和工作人员进行定性访谈.
  • 以规范化过程理论为指导的分析,以检查临床决策和协作.

主要成果:

  • 专业人士认为,在非流行病的一般实践中,没有临床需要使用POC PCR设备.
  • 虽然患者的结果更快,但全科医生经历了任务和工作量增加.
  • 节省时间并没有直接使全科医生受益.

结论:

  • 当前的POC PCR测试技术没有提供足够的诊断附加值,以证明日常一般实践中工作量增加的理由.
  • 需要解决与工作流程和感知到的临床实用性相关的实施挑战.
  • 需要进行进一步的研究,以优化POC PCR用于常规的全科医院环境.