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自相兼容性是在多倍化后获得的:Brassica napus自相不兼容的三线性杂交繁殖系统的案例研究

Shengwei Dou^1,2, Tong Zhang^2,3, Lulin Wang^1,2

¹National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.

The New phytologist

|December 1, 2023

概括

植物的自我不相容性在多化后转变为自我相容性. 在油菜种子中,像SMI和SMI2这样的S-locus小RNAs (sRNAs) 通过沉默SCR基因来调节这种过渡,从而实现针对期望特征的向繁殖.

科学领域:

植物生殖生物学植物生殖生物学
遗传学和基因组学遗传学和基因组学
分子进化是分子进化的过程.

背景情况:

自我不相容性 (SI) 对于 angiosperm 的遗传多样性至关重要.
聚化通常会导致从SI向自我兼容性 (SC) 的过渡.
S位点控制SI,但其在多倍体SC中的作用不太清楚.

研究的目的:

研究S-locus小RNA (sRNA) 位点,SMI和SMI2的起源和功能,在Brassicaceae中.
确定这些sRNAs通过哪些机制来影响油菜 (Brassica napus) 的自我相容性.
探索繁殖自我不兼容或自我兼容的菜种子系的潜力.

主要方法:

在Brassicaceae中的S位点区域的比较分析.
识别和描述SMI和SMI2的位置.
在Brassica napus中进行基因过度表达实验.
DNA甲基化分析.
分析sRNA介导的基因沉默.

主要成果:

确定了SMI (在Cruciferae中广泛存在) 和SMI2 (Brassica除外) 的不同来源.
在菜中发现了四种主要的S类型 (BnS-1,BnS-6,BnS-7,BnS-1300).
过度表达BnSMI-1诱导DNA甲基化和自我相容性.
BnSMI2-1300通过转录抑制抑制了BnSCR-7的表达.

结论:

拉皮种子的自我兼容性是由S-locus sRNA引导的SCR基因在多聚体化后的沉默介导的.
SMI和SMI2sRNA在调节SI-SC过渡中发挥着关键作用.
这种机制为培育量身定制的菜品种提供了基础,从而提高了异构利用率.

关键词:

布拉斯卡纳普斯 (Brassica napus) 是一种植物.通过DNA甲基化.微笑微笑微笑在Smi2上,你会看到Smi2.自相兼容性自相兼容性自我不相容性自我不相容性

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