通过使用压力积分来改善毛细血管电泳中的水力动力注射
Eric Tavares da Costa1, Claudimir Lucio do Lago1
1Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
Electrophoresis
|December 1, 2023
概括
在毛细血管电泳中进行液态动力注射期间监测压力,可实现精确的体积控制. 通过压力积分 (IoP) 将峰值区域正常化,可以显著提高注射可重现性和精度.
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 仪器化 仪器化 仪器化
背景情况:
- 在毛细血管电泳 (CE) 中的水力动力注射对于可复制样本的引入至关重要.
- 传统的注射方法可能会因为压力波动而变化.
- 精确控制注射量对于在CE中进行定量分析至关重要.
研究的目的:
- 为了验证海根-波西尤尔模型在CE中进行水力动力注入时的层流.
- 建立压力监测作为评估注射体积的可靠方法.
- 开发和演示基于压力监测的液压动力注射动态控制系统.
主要方法:
- 使用哈根-波西尤尔模型,分析水力动力注入过程中的压力转换.
- 使用一个压力阻抗传感器来监测样品瓶中的头空间空气压力.
- 实现一个微控制器控制的系统,带有周静电和电磁,用于动态压力调节.
- 通过压力积分 (IoP) 评估分析剂峰值面积的正常化,以纠正注射变化.
主要成果:
- 哈根-波西耶模型被证实对层流是有效的,即使在压力变化期间也是如此.
- 通过 IoP 的规范化显著降低了峰值区域的相对标准偏差 (RSD),相当于使用内部标准.
- 一个基于微控制器的系统成功地控制了各种压力和时间组合的水力动力注射.
- 开发的系统在各种注射条件下实现了2.7%的IoP RSD.
结论:
- 压力综合 (IoP) 监测是一种可靠的方法,用于评估在水力动力学CE中注入的体积.
- 基于 IoP 的动态注射的动态控制提供了更好的可重现性和精度.
- 这种方法为传统方法提供了具有成本效益的替代方案,用于在CE中精确的样本引入.
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