在非结构性蛋白6中氨基酸替代L232F被确定为B类MERS冠状病毒中可能存在的人类适应突变
Ray T Y So1,2, Daniel K W Chu1,3, Kenrie P Y Hui1
1School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.
Journal of virology
|December 1, 2023
概括
中东呼吸综合征冠状病毒 (MERS-CoV) nsp6蛋白的特定突变L232F增强了病毒复制. 这一发现表明MERS-CoV适应人类传播,并强调需要持续对驼进行监测.
科学领域:
- 病毒学 病毒学
- 动物传播疾病 动物传播疾病
- 病毒进化是病毒的进化
背景情况:
- 病毒宿主适应对于冠状病毒和流感等病毒的物种间传播至关重要.
- 虽然在流感中已知人类适应性突变,但它们在中东呼吸系统综合征冠状病毒 (MERS-CoV) 中尚未被确定,尽管它是从大驼传播的动物性传播.
研究的目的:
- 为了确定MERS-CoV.病毒中潜在的人类适应性突变.
- 调查已识别的突变在病毒适应和传播中的功能作用.
主要方法:
- 对B类MERS-CoV序列的分析,以确定反复发生的突变.
- 使用功能丧失逆遗传学方法来评估病毒性质.
- 评估病毒复制能力 in vitro,ex vivo (人类上呼吸道) 和 in vivo (老鼠肺).
主要成果:
- 在人类的MERS-CoV分离物中,在nsp6蛋白中的氨基酸替代物L232F多次被发现.
- nsp6 L232F突变显著增加了体外,体外和体内模型中的病毒复制能力.
- 这种突变在人类呼吸道组织和小鼠肺部表现出增强的病毒适应性.
结论:
- nsp6 L232F突变是一种潜在的适应性突变,可以促进MERS-CoV对人类的动物传播.
- 这项研究强调了MERS-CoV适应人类宿主的能力.
- 对驼种群中MERS-CoV的持续监测对于监测和预防未来的疫情至关重要.
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