肠病原性E.E.E.E. 肠病原性 大肠杆菌感染共同引起溶酶体外和性宿主细胞死亡
Raisa Shtuhin-Rahav1,2, Aaron Olender2,3, Efrat Zlotkin-Rivkin1,2
1Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, The Edmond J. Safra Campus-Givat Ram, Jerusalem, Israel.
mBio
|December 1, 2023
概括
肠病原性大肠杆菌 (EPEC) 操纵宿主细胞死亡和 lysosomal exocytosis (LE) 的作用. 控制细胞死亡的EPEC因子也调节LE,揭示了对感染和疾病至关重要的相互连接机制.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病变的发生和发病.
背景情况:
- 肠病原性大肠杆菌 (EPEC) 引起显著的胃肠炎,特别是在儿童中.
- EPEC利用III型分泌系统注入能调节宿主细胞功能的效应蛋白.
- 了解EPEC与宿主细胞的相互作用对于开发治疗策略至关重要.
研究的目的:
- 研究EPEC效应蛋白在调节宿主细胞死亡和 lysosomal exocytosis (LE) 中的作用.
- 为了确定效应因子介导的细胞死亡调制和LE诱导之间的相关性.
- 为了阐明细胞死亡和LE在EPEC病变的相互联系.
主要方法:
- 对EPEC效应蛋白 (EspF,Map,EspZ) 的分析及其对宿主细胞活性的影响.
- 在感染宿主细胞中评估 lysosomal 贩运和外细胞形成.
- 细胞死亡和LE中的效应因子功能之间的相关性研究.
主要成果:
- EPEC 效应蛋白 EspF 和 Map 促进线粒体细胞死亡,而 EspZ 则对抗.
- 这些效应体还调节 lysosomal exocytosis (LE),控制 lysosome 贩运到等离子体膜.
- 效应因子诱导/保护细胞死亡的能力与它们诱导LE的能力之间存在完全的相关性.
结论:
- 宿主细胞死亡和 lysosomal exocytosis 是由 EPEC 调节的相互连接的过程.
- EPEC 效应器在控制细胞死亡和LE方面发挥着双重作用.
- 了解LE在宿主细胞死亡中的参与对于破译EPEC感染机制和疾病进展至关重要.
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