实时定量PCR在眼样本中的诊断性能,用于各种类型的简单疹角膜炎亚型
Gautier Hoarau1, Oscar Haigh2, Christelle Vauloup-Fellous2,3
1Service d'Ophtalmologie, Hôpital Bicêtre, Université Paris-Saclay, CRMR OPHTARA , Le Kremlin Bicêtre, France.
Journal of clinical microbiology
|December 1, 2023
概括
眼样本的实时定量PCR (RT-qPCR) 有助于诊断简单疹角膜炎 (HSK). 对于上皮角膜炎的敏感性最高,在具有挑战性的病例中证实了HSK.
科学领域:
- 眼科医生 眼科 眼科
- 病毒学 病毒学
- 分子诊断学 分子诊断学
背景情况:
- 简单疹角膜炎 (HSK) 诊断严重依赖于临床表现.
- 生物确认对于管理复杂的HSK病例至关重要.
- 实时定量PCR (RT-qPCR) 提供了一种在眼部感染中检测病毒的潜在方法.
研究的目的:
- 评估RT-qPCR对眼样本的有用性,以诊断HSK.
- 将RT-qPCR结果与HSK的临床诊断进行比较.
- 为了确定RT-qPCR在各种HSK亚型中的敏感性和特异性.
主要方法:
- 从2013年至2021年间对364名患者的临床记录和样进行了回顾性审查.
- 眼样本使用RT-qPCR检测单纯疹病毒1型.
- 临床诊断是基于生物显微镜发现和患者病史.
主要成果:
- 在465个样本中,240个具有活跃的HSK;76个是RT-qPCR阳性 (31.6%的灵敏度,99.5%的特异性).
- 在上皮角膜炎 (97.4%) 和带的 stromal 角膜炎 (48.7%) 中,RT-qPCR 敏感度最高.
- 在没有上皮干涉的HSK中,病毒载荷明显较低.
结论:
- 眼样本上的RT-qPCR可以证实所谓的HSK,即使没有明显的上皮参与.
- 通过RT-qPCR取样是非常敏感的,当上皮质角膜炎存在.
- 这种方法支持管理HSK具有挑战性的案件.
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