评估用于常规临床分析的五种多类固醇LC-MS/MS方法:九种分析物获得了可比性能
Valentin Braun1,2, Uta Ceglarek3, Alexander Gaudl3
1Institute of Pharmacy/Pharmacognosy, CCB - Centrum of Chemistry and Biomedicine, University of Innsbruck, Innsbruck, Austria.
Clinical chemistry and laboratory medicine
|December 1, 2023
概括
这项研究比较了五个实验室的类固醇测量结果,使用液体染色学-并联质谱法 (LC-MS/MS). 虽然总体上可以比较,但在某些类固醇中发现了偏差,这凸显了与熟练测试一起进行方法间比较的必要性.
科学领域:
- 临床化学 临床化学
- 质谱测量质量谱测量
- 类固醇分析 类固醇分析
背景情况:
- 准确的类固醇量化对于临床诊断至关重要.
- 实验室间比较对于评估试验性能和可比性至关重要.
- 液体染色学-双重质谱法 (LC-MS/MS) 是一种常见的类固醇分析方法.
研究的目的:
- 为了评估LC-MS/MS类固醇测量在多个实验室的可比性.
- 确定类固醇量化中的潜在分析挑战和偏见.
- 为了证明外部质量评估的方法间比较的价值.
主要方法:
- 一个基于质谱 (LC-MS/MS) 的实验室间比较研究,涉及五个实验室.
- 使用组合的人类血清样本对9种类固醇分析物的分析.
- 参与者使用了自己的多分析仪协议和校准器,测量平台和测量套件的变化.
主要成果:
- 对于大多数类固醇分析物来说,观察到良好至可接受的整体可比性.
- 平均偏差通常在±10%以内,除了某些部位的脱水氨二硫酸 (DHEAS),安德罗二和孕激素.
- 识别的分析问题包括以前未被识别的偏差,操作员错误,矩阵效应以及较低测量范围的精度/不精度降低.
结论:
- 方法间比较对于监测试验有效性至关重要.
- 这项研究作为外部质量评估的模型,补充了能力测试计划.
- 标准化的实验室间比较可以提高类固醇测量的可靠性.
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