Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.4K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.4K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.4K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

9.2K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.2K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.5K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.5K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Responses of <i>Coix lacryma-jobi</i> L. to Exogenous Phenolic Acid Treatments: Effects on Growth, Antioxidant Responses, and Leaf Metabolome.

Plants (Basel, Switzerland)·2026
Same author

Effects of Different Selenium Concentrations on Agronomic Traits, Antioxidant Defense, and Leaf Metabolome in Blueberry (<i>Vaccinium corymbosum</i> L. 'Brigitta').

Plants (Basel, Switzerland)·2026
Same author

AI-guided design and optimization of a novel KIM-1-targeted peptide for bFGF delivery in acute kidney injury repair.

Regenerative biomaterials·2026
Same author

Associations between various lipid indices and coronary collateral circulation in patients with acute ST-segment elevation myocardial infarction: a cross-sectional study.

International journal of cardiology. Cardiovascular risk and prevention·2026
Same author

Interchain supramolecular interactions drive nearly 21% efficiency organic solar cells.

Nature communications·2026
Same author

Glutamine promotes acute wound healing by mediating glutamine metabolism and M2 macrophage polarization via the MEK/ERK/SLC1A5 signaling pathway.

Scientific reports·2026

相关实验视频

Updated: Jul 9, 2025

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
11:14

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope

Published on: May 28, 2016

13.9K

一种增强的可视化图像采集方法,用于在常规扫描电子显微镜下传导性差的样品.

Shuiquan Pang1, Hao Xia1, Xianmin Zhang2

  • 1China Electronic Product Reliability and Environmental Testing Research Institute, Guangzhou, Guangdong 511370, People's Republic of China.

The Review of scientific instruments
|December 1, 2023
PubMed
概括

使用扫描电子显微镜 (SEM) 分析导电性较差的样本可以掩盖细节. 本研究引入了一种使用图像注册和多传感器融合的增强可视化方法,以揭示更清晰的微观结构信息.

更多相关视频

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
07:33

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

10.6K
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.5K

相关实验视频

Last Updated: Jul 9, 2025

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope
11:14

Comprehensive Characterization of Extended Defects in Semiconductor Materials by a Scanning Electron Microscope

Published on: May 28, 2016

13.9K
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
07:33

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

10.6K
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
11:19

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

10.5K

科学领域:

  • 材料科学 材料科学 材料科学
  • 显微镜的使用方法
  • 图像分析 图像分析

背景情况:

  • 传统的扫描电子显微镜 (SEM) 模式,如低真空和低加速电压,对于分析导电性差的样品是非常实用的.
  • 然而,这些模式可以掩盖或消除关键的结构特征信息,阻碍对局部微观结构的分析.
  • 这种局限性在准确地描述导电性差的材料方面构成了重大挑战.

研究的目的:

  • 开发一个增强的可视化图像采集方法,用于导电性较差的样品.
  • 克服传统的SEM成像模式在揭示微观结构细节方面的局限性.
  • 为从具有挑战性的样本中获得更清晰的成像数据提供一种新的方法.

主要方法:

  • 拟议的方法使用图像注册技术.
  • 采用多传感器融合技术,结合来自不同来源的数据.
  • 这种方法旨在提高在导电性差的样本中结构特征的可视化.

主要成果:

  • 与标准的SEM图像相比,增强的可视化方法成功生成了具有更清晰地形信息的图像.
  • 实验结果证明了拟议技术在克服信息丢失方面的有效性.
  • 该方法为微观结构的分析提供了改进的视觉数据.

结论:

  • 开发的方法为在SEM中分析导电性较差的样品提供了显著的改进.
  • 通过图像注册和微观结构识别和测量中的多传感器融合辅助来增强可视化.
  • 这种技术为研究人员在具有挑战性的材料中研究微观结构提供了有价值的新参考.