循环脂使原细胞生命周期成为可能.
Ö Duhan Toparlak1, Lorenzo Sebastianelli2, Veronica Egas Ortuno3
1Department of Cellular, Computational and Integrative Biology, University of Trento, Via Sommarive 9, 38123 Povo, Trentino, Italy.
ACS nano
|December 1, 2023
概括
由环脂和脂肪酸制成的新原细胞可以生长和分裂,保留遗传物质. 这一突破解决了生命起源研究的一个关键挑战,使得达尔文进化在前生物条件下成为可能.
科学领域:
- 生命的起源 研究 研究 研究
- 益生菌化学 益生菌化学
- 生物化学 生物化学
背景情况:
- 目前使用脂肪酸囊泡的原细胞模型在核酸功能所需的度下失败.
- 现有的改善囊泡稳定性的方法,如益生菌合物,阻碍原细胞生长和分裂.
- 由于这些局限性,自我复制原细胞出现的合理途径仍然难以捉摸.
研究的目的:
- 开发一种能够在必需离子的存在下生长和分裂的前生物可信的原细胞模型.
- 为了证明在原细胞复制过程中封装核酸的保留.
- 为了证明与早期基因组复制所需的关键化学过程的兼容性.
主要方法:
- 从循环脂和脂肪酸的混合物构建原细胞.
- 在Mg2+-酸盐的存在下测试原细胞的稳定性和功能.
- 评估核酸保留,核酸吸收以及与非酶性RNA扩展的兼容性.
主要成果:
- 由环脂和脂肪酸混合物组成的原细胞成功生长并分裂为Mg2+-酸盐.
- 封装核酸在整个生长和分裂周期中被保留.
- 原细胞表现出获取周围核酸的能力,并支持非酶性RNA寡核酸扩展.
结论:
- 循环脂和脂肪酸的脂质混合物形成了在益生菌条件下活跃的强壮原细胞.
- 这些原细胞克服了先前模型的关键限制,支持核酸功能和复制.
- 这些发现为达尔文进化论从无生命物质的出现提供了一个可行的途径.
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