由乳球菌乳酸菌和Pediococcus pentosaceus菌株产生的抗微生物 (细菌素),具有对临床和食源性病原体的活性
Gee Hyeun Choi1, Joanna Ivy Irorita Fugaban1,2, Clarizza May Dioso3,4
1ProBacLab, Department of Advanced Convergence, Handong Global University, 37554, Pohang, Gyeongbuk, Republic of Korea.
Probiotics and antimicrobial proteins
|December 1, 2023
概括
从韩国发酵食品中分离出来的乳酸细菌 (LAB) 产生细菌素,这些细菌素是抗微生物,在食品保存和制药方面具有潜在的应用. 这些细菌素表现出稳定性和有效性,对抗致病细菌,如李斯特菌和葡萄球菌.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 细菌素是细菌产生的抗微生物,特别是乳酸细菌 (LAB).
- 实验室细菌素表现出广泛的抑制,这表明在食品防腐和制药领域的应用.
- 鉴定和描述新型产生细菌素的LAB对于探索它们的潜力至关重要.
研究的目的:
- 从Pohang,韩国的发酵食品和水果中分离和识别产生细菌素的LAB.
- 评估有希望的LAB分离物的细菌素生产,稳定性和粘附性质.
- 调查细菌菌素基因在已识别的菌株中的存在及其对病原性细菌的有效性.
主要方法:
- 使用生理,生化和分子方法 (16S rRNA测序) 隔离和识别LAB.
- 细菌素的生产,稳定性 (酶,化学物质,pH,温度) 和粘附性质的评估.
- 用PCR分析检测细菌素基因 (尼辛操作子,小儿素PA-1操作子) 并评估它们对Listeria spp.的有效性. 和葡萄球菌 spp.
主要成果:
- 他们发现了三个有前途的LAB菌株:Pediococcus pentosaceus 732,Lactococcus lactis 808和Lactococcus lactis亚种. 牛奶公司 431. lactis 431.
- 这些菌株产生的细菌素在各种条件下表现出稳定性和粘附性.
- 通过PCR检测证实了乳球菌菌株中尼操作子 (具有突变) 的存在,以及P. pentosaceus 732中的小儿素PA-1操作子.
- 这些细菌素对Listeria spp.表现出抗菌作用. 和葡萄球菌 spp.
结论:
- 已确定的LAB菌株,特别是Pediococcus pentosaceus 732和Lactococcus lactis菌株,是新型细菌素的潜在来源.
- 这些细菌素具有理想的稳定性和抗微生物特性,使其适用于食品防腐和制药应用.
- 对细菌素生产的具体机制和优化进行进一步研究是有必要的.
更多相关视频
06:05Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
Published on: September 30, 2014
13.6K
10:13Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
11.9K
相关概念视频
01:23Antimicrobial Proteins
1.0K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.0K
01:18Surface Membrane Barriers
1.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.1K
01:31Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K