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激情果的综合性多学分析揭示了水果颜色和香味的遗传基础

Yu-Yu Zheng^1,2, Lin-Hua Chen¹, Bing-Liang Fan¹

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China.

Plant physiology

|December 1, 2023

概括

组装了激情水果基因组,揭示了最近的重复和变异. 这项研究揭示了激情果品种中独特的风味和香味的遗传和代谢基础.

科学领域:

基因组学就是基因组学.
植物科学植物科学
代谢学代谢学代谢学

背景情况:

激情果 (Passiflora edulis) 是一种以其复杂的香味而闻名的全球种植作物.
了解其独特的风味和香味特征的遗传基础对于作物改进至关重要.

研究的目的:

执行新的基因组组装和对紫色激情果 (PPF) 和黄色激情果 (YPF) 的比较分析.
确定导致不同品种激情果的独特风味,香气和颜色特征的遗传和代谢因素.

主要方法:

利用PacBio,Illumina和Hi-C测序进行新的基因组组装和注释.
进行了比较基因组学,转录组学和代谢组学分析.
在酵母 (Saccharomyces cerevisiae) 中进行了合成酶 (TPS) 的异质表达.

主要成果:

成功组装和注释PPF和YPF参考基因组,识别最近的全基因组重复事件.
发现了包括SNP,InDels在内的显著基因组变异,以及品种之间的存在/缺席变异.
确定了关键的代谢途径 (黄类,类,类) 和候选基因,与激情果的颜色和香味有关,类在PPF中积累更多.
从功能上描述了12个烯合成酶,揭示了YPF和PPF之间的保留和分离功能.

结论:

这项研究阐明了基因组架构和基因变异是激情果多样性的基础.
确定了PPF和YPF品种中独特的香味和风味配置文件的遗传和代谢基础.
提供了宝贵的基因组资源,以加速激情果的遗传改进和繁殖.

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