在玉米中全基因组识别通道显示了进化模式和对非生物应激的可变功能反应
Mallana Gowdra Mallikarjuna1, Rakhi Tomar1, Hirenalluru Chandappa Lohithaswa2
1Division of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi, 110012, India.
Plant physiology and biochemistry : PPB
|December 1, 2023
概括
玉米的路对于植物的抗压能力至关重要. 基因重复和净化选择塑造了这些必不可少的K +通道,具有应对干旱和水淹没的特定调节元素.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- (K +) 通道对于植物K + 恒温,生理过程和耐压力至关重要.
- 了解K+通道的演变和调节是提高作物弹性的关键.
研究的目的:
- 在玉米中识别和描述K+通道.
- 研究K+通道基因的进化模式和染色体分布.
- 探索在压力下控制K+通道表达的调节元件和网络.
主要方法:
- 在单种植物和双种植物之间进行比较的基因组学.
- 染色体定位和基因复制分析.
- 为进化见解计算Ka/Ks比率.
- 对于cis-regulatory元素的推动者分析.
- 定量实时PCR (qRT-PCR) 用于基因表达分析.
主要成果:
- 在玉米中确定了27个K+通道,染色体分布不均.
- 分散重复是K+通道基因扩张的主要驱动因素.
- 观察到强烈的净化选择,在相关物种中具有突出的一对一的正义学.
- 确定MYB和STRE结合点是应激耐受性的关键 cis-调节元素.
- AP2-EREBP TFs,miR164和miR399被确定为重要的监管因素.
- 在干旱和淹水的情况下,K+通道和调节性miRNAs显示出显著的表达变化.
结论:
- 玉米K+通道经历了由基因重复和净化选择驱动的显著扩张和多样化.
- 特定的cis-regulatory元素和miRNA途径对于K+通道介导的应激反应至关重要.
- 这项研究为未来对玉米K+通道在应力适应中的功能研究提供了基础.
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