关于子嘴颜色的改变纪律的研究,以及基于转录组测序的调节网络
Rui Pan1, Tian Hua1, Yifan Ding1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Poultry science
|December 1, 2023
概括
子嘴的颜色在化后发生了显著的变化,黑色的嘴可能涉及黄色素沉积. EDNRB信号通路和TYR等基因对于尾颜色的发展至关重要.
科学领域:
- 动物遗传学动物遗传学
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 嘴巴颜色是子品种的一个关键特征,黑色的嘴巴很少见,而且很可取.
- 了解子颜色的发育对于识别遗传资源和高质量的产品很重要.
研究的目的:
- 为了研究嘴颜色的发育变化.
- 识别与的颜色相关的遗传标记和监管网络.
- 为了阐明子黑嘴形成背后的分子机制.
主要方法:
- 伦州白创造 (黑嘴) 和普蒂安黑 (黄嘴) 的杂交,创造了一代F2.
- 随着时间的推移,鼻子颜色变化的表型观测和统计分析.
- 转录组测序以确定差异表达的基因.
- 权重基因同表达网络分析 (WGCNA) 和趋势分析.
- 实时定量聚合酶连锁反应 (RT-qPCR) 用于验证.
主要成果:
- 在F1和F2世代从化到42天观察到显著的巴颜色变化.
- 在F2中,近一半的黑鼻幼发育了绿色的嘴巴;灰色和有图案的嘴巴随着年龄的增长显示出越来越多的黑色斑点.
- 从第一天开始,嘴巴的颜色变暗,从第二天开始,灰色的颜色值显著下降.
- 转录组分析显示,在4-6周后,黑色和黄色的之间TYR的差异表达.
- 在第一个星期,EDNRB信号通路基因和MITF的高度表达,而TYR,TYRP1和DCT在4-6周后的高度表达.
结论:
- 嘴颜色在化后发生动态变化,涉及黑色素合成和潜在的黄色色素沉积.
- 该EDNRB信号通路可能在早期黑色素体成熟和黑色素形成中发挥作用.
- 像TYR这样的基因对于在子中维持黑孔表型至关重要.
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